Liu Jun-Qiu, Si Nan, Yang Jian, Zhao Hai-Yu, Bian Bao-Lin, Wang Hong-Jie
Yao Xue Xue Bao. 2014 Feb;49(2):244-8.
Cinobufacino injection is a significant anti-tumor medicine for the treatment of various tumors in clinic, which was made from water extraction of the skin of Bufo bufo gargarizans. In present paper, HPLC-DAD-FT-ICR-MS method was used to identify the major bufadienolides in cinobufacino for the first time. Solid-phase extraction with dichloromethane and silica was used to enrich the total bufadienolides in cinobufacino. Based on the UV and high resolution MS/MS data, 33 bufadienolides were analyzed and characterized. Among them, eight compounds were identified by comparing with standard references unambiguously. This study elucidated the major bufadienolides in cinobufacino, which provided material foundation of cinobufacino and will be benefit for the further pharmacological research.
华蟾素注射液是临床上治疗多种肿瘤的一种重要抗肿瘤药物，它由中华大蟾蜍皮肤水提取物制成。在本文中，首次采用高效液相色谱-二极管阵列检测-傅里叶变换离子回旋共振质谱法对华蟾素中的主要蟾毒配基进行鉴定。采用二氯甲烷和硅胶固相萃取法富集华蟾素中的总蟾毒配基。基于紫外和高分辨串联质谱数据，对33种蟾毒配基进行了分析和表征。其中，通过与标准对照品比较，明确鉴定出8种化合物。本研究阐明了华蟾素中的主要蟾毒配基，为华蟾素提供了物质基础，将有助于进一步的药理研究。
