Rezaeifard Somayeh, Razmkhah Mahboobeh, Robati Minoo, Momtahan Mozhdeh, Ghaderi Abbas
Shiraz Institute for Cancer Research, Departments of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
J Cancer Res Ther. 2014 Jan-Mar;10(1):159-64. doi: 10.4103/0973-1482.131451.
The main site of ovarian cancer metastasis is the omentum. Omental adipose tissue is known for contribution to the tumor growth and metastasis through different mechanisms.
In the present study, adipose derived stem cells (ASCs) were isolated from the omentum of patients with ovarian cancer and those with ovarian cysts and the expression of chemokines, chemokine receptors and cytokines were analyzed.
ASCs were isolated from omental adipose tissues obtained of 10 ovarian cancer and 25 ovarian benign cyst patients. Our investigations were done by quantitative real time-polymerase chain reaction, flowcytometry, western blot and also enzyme-linked immunosorbent assay.
Expression of CXCL-10 and CCR5 showed statistically significant difference between omentum derived ASCs of ovarian cancer patients compared with those with benign cysts (P < 0.05). Expression of interleukin-10 also detected in the supernatant of cultured malignant ASCs.
Omental adipose tissue may play crucial roles for tumor promotion through the expression of tumor promoting chemokines. Accordingly, tumor surrounding adipose tissue may be a novel target for immunotherapy of cancer.
卵巢癌转移的主要部位是大网膜。大网膜脂肪组织通过不同机制促进肿瘤生长和转移。
在本研究中，从卵巢癌患者和卵巢囊肿患者的大网膜中分离脂肪来源干细胞（ASC），并分析趋化因子、趋化因子受体和细胞因子的表达。
从10例卵巢癌患者和25例卵巢良性囊肿患者的大网膜脂肪组织中分离ASC。我们通过定量实时聚合酶链反应、流式细胞术、蛋白质印迹法以及酶联免疫吸附测定法进行研究。
与良性囊肿患者相比，卵巢癌患者大网膜来源的ASC中CXCL-10和CCR5的表达存在统计学显著差异（P < 0.05）。在培养的恶性ASC的上清液中也检测到白细胞介素-10的表达。
大网膜脂肪组织可能通过表达促肿瘤趋化因子在肿瘤促进中发挥关键作用。因此，肿瘤周围脂肪组织可能是癌症免疫治疗的新靶点。
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