Sadegh Amin Bigham, Basiri Ehsan, Oryan Ahmad, Mirshokraei Pezhman
Department of Veterinary Surgery and Radiology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran,
Cell Tissue Bank. 2014 Mar;15(1):127-37. doi: 10.1007/s10561-013-9383-z. Epub 2013 Jun 21.
Adipose derived adult stem cells (ASCs) are multipotent cells that are able to differentiate into osteoblasts in presence of certain factors. The histological characteristics of periosteum makes it a specific tissue with a unique capacity to be engineered. Higher flexibility of the greater omentum is useful for reconstructive surgery. These criteria make it suitable for tissue engineering. The present study was designed to evaluate bone tissue engineering with periosteal free graft concurrent with ASCs and pedicle omentum in dog model. Twelve young female indigenous dogs were used in this experiment. In omental group (n = 4), end of omentum was wrapped by periosteum of the radial bone in abdomen of each dog. In omental-autogenously ASCs group (n = 4), 1 ml of ASCs was injected into the wrapped omentum with periosteum while in omental-allogenously ASCs group (n = 4), 1 ml of allogenous ASCs was injected. Lateral view radiographs were taken from the abdominal cavity postoperatively at the 2nd, 4th, 6th and 8th weeks post-surgery. Eight weeks after operation the dogs were re-anesthetized and the wrapped omenum by periosteum in all groups was found and removed for histopathological evaluation. Our results showed that omentum-periosteum, omental-periosteum-autogenous ASCs and omental-periosteum-allogenous ASCs groups demonstrated bone tissue formation in the abdominal cavity in dog model. The radiological, macroscopical and histological findings of the present study by the end of 8 weeks post-surgery indicate bone tissue engineering in all three groups in an equal level. The present study has shown that the wrapped omentum with periosteum concurrent with ASCs (autogenous or allogenous ASCs) lead to a favorable bone tissue formation. We suggested that it may be useful when pedicle graft omentum used concurrent with periosteum in the bone defect reconstruction, and this phenomenon should be studied in future.
脂肪来源的成体干细胞（ASCs）是多能细胞，在某些因子存在的情况下能够分化为成骨细胞。骨膜的组织学特征使其成为具有独特工程化能力的特定组织。大网膜更高的柔韧性对重建手术有用。这些标准使其适用于组织工程。本研究旨在评估在犬模型中，将无蒂骨膜移植物与ASCs及带蒂大网膜同时应用于骨组织工程的效果。本实验使用了12只年轻的雌性本地犬。在大网膜组（n = 4）中，将每只犬腹部桡骨的骨膜包裹在大网膜末端。在大网膜-自体ASCs组（n = 4）中，将1毫升ASCs注入包裹有骨膜的大网膜中，而在大网膜-异体ASCs组（n = 4）中，注入1毫升异体ASCs。术后第2、4、6和8周从腹腔拍摄侧位X线片。术后8周，再次对犬进行麻醉，找到并取出所有组中被骨膜包裹的大网膜，进行组织病理学评估。我们的结果表明，大网膜-骨膜组、大网膜-骨膜-自体ASCs组和大网膜-骨膜-异体ASCs组在犬模型的腹腔中均显示有骨组织形成。本研究术后8周结束时的放射学、大体和组织学结果表明，三组中的骨组织工程水平相当。本研究表明，包裹有骨膜并同时应用ASCs（自体或异体ASCs）的大网膜可导致良好的骨组织形成。我们建议，在骨缺损重建中，当带蒂大网膜移植物与骨膜同时使用时可能会有用，并且这种现象应在未来进行研究。
