Li Chun-Hong, Fu Rong, Wang Yi-Hao, Song Wen-Jing, Ruan Er-Bao, Qu Wen, Wang Hua-Quan, Wang Guo-Jin, Song Jia, Wang Xiao-Ming, Wu Yu-Hong, Xing Li-Min, Liu Hong, Li Li-Juan, Guan Jing, Shao Zong-Hong
Department of Hematology, Tianjin Medical University General Hospital, Tianjin 300052, China.
Department of Hematology, Tianjin Medical University General Hospital, Tianjin 300052, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Apr;22(2):339-43. doi: 10.7534/j.issn.1009-2137.2014.02.014.
This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma (DLBCL) paraffin-embedded tissues, and evaluate its potential relevance with clinical characteristics. The expression levels of miR-21 in 26 primary DLBCL and 10 normal lymph node tissue specimens were examined by real-time polymerase chain reaction. The expression of PTEN was detected by immunohistochemical staining. The results indicated that the expression of miR-21 was significantly higher in tumor tissues [6.586(1.10,38.22)] than that in normal tissues [0.791 (0.35,2.87)] (P < 0.05). Among 26 patients with DLBCL the expression of PTEN protein was positive in 6 patients (23%), and was negative in 20 patients (77%). In patients with DLBCL, the expression level of miR-21 was negatively correlated with the level of PTEN protein. The high expression of miR-21 was positively correlated with the level of serum LDH. The expression level of miR-21 in patients with Ann Arbor III-IV stage was obviously higher than that of patients with Ann Arbor I-II stage, but did not correlate with the subtype of patients in clinic (P > 0.05). It is concluded that the expression of miR-21 is high in DLBCL and its overexpression may be related with poor prognosis of DLCBL. These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL.
本研究旨在探讨微小RNA-21（miR-21）在弥漫性大B细胞淋巴瘤（DLBCL）石蜡包埋组织中的表达及其与PTEN的相关性，并评估其与临床特征的潜在关联。采用实时聚合酶链反应检测26例原发性DLBCL和10例正常淋巴结组织标本中miR-21的表达水平。通过免疫组织化学染色检测PTEN的表达。结果表明，肿瘤组织中miR-21的表达[6.586(1.10,38.22)]显著高于正常组织[0.791(0.35,2.87)]（P<0.05）。26例DLBCL患者中，PTEN蛋白表达阳性6例（23%），阴性20例（77%）。在DLBCL患者中，miR-21的表达水平与PTEN蛋白水平呈负相关。miR-21的高表达与血清乳酸脱氢酶水平呈正相关。Ann Arbor III-IV期患者中miR-21的表达水平明显高于Ann Arbor I-II期患者，但与患者的临床亚型无关（P>0.05）。结论：DLBCL中miR-21表达较高，其过表达可能与DLCBL预后不良有关。这些发现提示PTEN可能是DLBCL中miR-21的靶标之一。
