Li Xin, Wang Fang, Ding Kai Yang, Dai Lan
Department of Hematology,Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China.
Department of Hematology,Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Apr;22(2):403-6. doi: 10.7534/j.issn.1009-2137.2014.02.025.
This study was aimed to investigate the role of regulatory B cells (Breg) in pathogenesis of immune thrombocytopenia (ITP) and its clinical significance. A total of 35 ITP patients and 20 normal controls were enrolled in this study. The expression of CD19(+)CD24(hi)CD38(hi) B cells was detected by flow cytometry and the expression of IL-10 mRNA and TGF-β1 mRNA was assayed by RT-PCR. The results indicated that the expression level of CD19(+)CD24(hi)CD38(hi) B cells in peripheral blood of newly diagnosed ITP patients was obviously lower than that in normal controls (P < 0.05); the expression level of CD19(+)CD24(hi)CD38(hi) B cells in ITP patients with increased platelet count after treatment was higher than that before treatment (P < 0.05); the expression level of IL-10 mRNA in newly diagnosed ITP patients was significantly lower than that the in normal controls (P < 0.05), the expression level of TGF-β1 mRNA in newly diagnosed ITP patients increases as compared with normal controls (P < 0.05), after treatment with DXM the expression of IL-10 mRNA was enhanced, the expression of TGF-β1 mRNA was reduced as compared with expression level before treatment (P < 0.05). It is concluded that the Breg cells may play an important role in the pathogenesis of ITP via humoral immunity and its regulation of T lymphocytes.
本研究旨在探讨调节性B细胞（Breg）在免疫性血小板减少症（ITP）发病机制中的作用及其临床意义。本研究共纳入35例ITP患者和20例正常对照。采用流式细胞术检测CD19(+)CD24(hi)CD38(hi) B细胞的表达，采用RT-PCR检测IL-10 mRNA和TGF-β1 mRNA的表达。结果显示，初诊ITP患者外周血中CD19(+)CD24(hi)CD38(hi) B细胞的表达水平明显低于正常对照（P < 0.05）；治疗后血小板计数升高的ITP患者CD19(+)CD24(hi)CD38(hi) B细胞的表达水平高于治疗前（P < 0.05）；初诊ITP患者IL-10 mRNA的表达水平显著低于正常对照（P < 0.05），初诊ITP患者TGF-β1 mRNA的表达水平较正常对照升高（P < 0.05），地塞米松治疗后IL-10 mRNA的表达增强，TGF-β1 mRNA的表达较治疗前降低（P < 0.05）。结论：Breg细胞可能通过体液免疫及其对T淋巴细胞的调节在ITP发病机制中发挥重要作用。
