Ambler J
Clinical Chemistry Department, University Hospital, Queen's Medical Centre, Nottingham, England.
Electrophoresis. 1989 Jul;10(7):520-3. doi: 10.1002/elps.1150100712.
The method of isoelectric focusing proteins on cellulose acetate membranes has been improved by the introduction of: (i) plastic-backed membranes, (ii) a commercially available Mini-IEF cell and (iii) sensitive colloidal gold staining. The improved methodology is described with applications for clinical laboratories.