通过固定化pH梯度等电聚焦法测定α-1-抗胰蛋白酶表型和M亚型
Determination of alpha-1-antitrypsin phenotypes and M subtypes by isoelectric focusing in immobilized pH gradients.
作者信息
Brooks K P, Iammarino R M
出版信息
Clin Biochem. 1985 Oct;18(5):280-4. doi: 10.1016/s0009-9120(85)80032-1.
A procedure to determine alpha-1-antitrypsin phenotypes (Pi types), including the six common PiM subtypes, is described. Phenotypes of the inhibitor, including the deficiency phenotype PiZ and the PiM subtypes, can be identified by isoelectric focusing in an immobilized pH gradient with a pH range of 4.2 to 5.0 or 4.0 to 5.0. This method gives clear separation of M, S, V, P and Z allele products. Up to 24 samples can be analyzed in a single overnight run using commercially available equipment and reagents.
本文描述了一种用于确定α-1-抗胰蛋白酶表型（Pi型）的方法，其中包括六种常见的PiM亚型。通过在固定pH梯度（pH范围为4.2至5.0或4.0至5.0）中进行等电聚焦，可以识别抑制剂的表型，包括缺陷型PiZ和PiM亚型。该方法能够清晰分离M、S、V、P和Z等位基因产物。使用市售设备和试剂，在单次过夜运行中最多可分析24个样本。
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