Thiriart C, Francotte M, Cohen J, Collignon C, Delers A, Kummert S, Molitor C, Gilles D, Roelants P, van Wijnendaele F
Department of Molecular and Cellular Biology, Smith Kline-RIT, Rixensart, Belgium.
J Immunol. 1989 Sep 15;143(6):1832-6.
Mouse mAb reactive to the HIV-1 envelope glycoprotein precursor gp160 of the HTLVIII(B) isolate were characterized in radioimmunoprecipitation and immunoblot tests with the use of HTLVIII(B) isolate as Ag. The reactivities of these mAb were also measured in a capture enzyme immunoassay and in radioimmunoprecipitation assay by using gp160 and gp120 expressed as vaccinia recombinants. Striking differences in exposure of specific epitopes were noted between the gp120 component of the gp160 precursor and the fully processed gp120 in both tests. These conformational rearrangements affecting the gp120 moiety of the HIV-1 envelope glycoprotein might have important implications on its immunogenicity.
利用HTLVIII(B)分离株作为抗原，通过放射免疫沉淀和免疫印迹试验对与HTLVIII(B)分离株的HIV-1包膜糖蛋白前体gp160反应的小鼠单克隆抗体（mAb）进行了表征。还通过使用痘苗重组体表达的gp160和gp120，在捕获酶免疫测定和放射免疫沉淀测定中测量了这些mAb的反应性。在这两种试验中，gp160前体的gp120组分与完全加工的gp120之间在特定表位的暴露方面存在显著差异。这些影响HIV-1包膜糖蛋白gp120部分的构象重排可能对其免疫原性具有重要意义。
