Murphy M, Schenk P, Lankinen H M, Cross A M, Taylor P, Owsianka A, Hope R G, Ludwig H, Marsden H S
MRC Virology Unit, University of Glasgow, U.K.
J Gen Virol. 1989 Sep;70 ( Pt 9):2357-64. doi: 10.1099/0022-1317-70-9-2357.
Previously we have described the isolation of seven monoclonal antibodies (MAbs) and two polyvalent rabbit sera directed against the product of herpes simplex virus type 1 (HSV-1) gene UL42, a 65K DNA-binding protein (65KDBP) which is essential for HSV DNA replication and virus growth. We now report the synthesis of all 483 overlapping hexapeptides of this 488 amino acid protein and describe their use for the identification of epitopes recognized by these MAbs and polyvalent sera. MAb 6898, derived from one fusion, recognizes the peptides EDLDGA and DLDGAA which correspond to amino acids 363 to 369 of 65KDBP. MAbs Z4D4, Z6F3, Z1A8, Z10Cl, Z3H12 and Z1F11, derived from a second fusion, all recognize the peptides GDPEDL and DPEDLD which correspond to amino acids 360 to 366. As expected both polyvalent sera recognize several different epitopes.
此前我们已经描述了针对单纯疱疹病毒1型（HSV-1）基因UL42产物的七种单克隆抗体（MAb）和两种多价兔血清的分离情况，该产物是一种65K DNA结合蛋白（65KDBP），对HSV DNA复制和病毒生长至关重要。我们现在报告了这种488个氨基酸的蛋白质的所有483个重叠六肽的合成情况，并描述了它们在鉴定这些单克隆抗体和多价血清所识别的表位中的用途。源自一次融合的单克隆抗体6898识别肽EDLDGA和DLDGAA，它们对应于65KDBP的氨基酸363至369。源自第二次融合的单克隆抗体Z4D4、Z6F3、Z1A8、Z10C1、Z3H12和Z1F11均识别肽GDPEDL和DPEDLD，它们对应于氨基酸360至366。正如预期的那样，两种多价血清都识别几种不同的表位。
