Bjelke B, Seiger A
Department of Histology, Karolinska Institutet Stockholm, Sweden.
Neurosci Lett. 1989 Jul 17;102(1):15-9. doi: 10.1016/0304-3940(89)90300-5.
Substance P (SP)-immunoreactive cell groups from fetal rat brain were homografted to the anterior eye chamber and left there for 2 months to mature, to elucidate their capacity to form nerve fibers in this ectopic environment. All grafts survived well, were rapidly revascularized from the host iris and proliferated to final sizes 2- to 4-fold the volume at transplantation. In spite of a rich SP fiber innervation of the transplant neuropil, no or almost no SP-positive fibers reached out from the transplant on to the iris. Monoamine histochemical analysis of the same locus coeruleus transplants revealed abundant fluorescent locus-derived nerve fibers of the host irides. Thus, a marked difference in growth behavior between noradrenergic and SP-immunoreactive neurons from the same brainstem region was found.
将来自胎鼠脑的P物质（SP）免疫反应性细胞群移植到眼前房，并在那里放置2个月使其成熟，以阐明它们在这种异位环境中形成神经纤维的能力。所有移植物存活良好，迅速从宿主虹膜获得血管再生，并增殖到移植时体积的2至4倍。尽管移植的神经毡有丰富的SP纤维神经支配，但没有或几乎没有SP阳性纤维从移植物延伸到虹膜上。对同一蓝斑移植物进行的单胺组织化学分析显示，宿主虹膜中有大量来自蓝斑的荧光神经纤维。因此，发现来自同一脑干区域的去甲肾上腺素能神经元和SP免疫反应性神经元在生长行为上存在显著差异。
