Piras Cristina, Scano Paola, Locci Emanuela, Sanna Roberta, Marincola Flaminia Cesare
Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella di Monserrato, SS 554, 09042 Monserrato, CA, Italy.
Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella di Monserrato, SS 554, 09042 Monserrato, CA, Italy.
Food Chem. 2014 Sep 15;159:71-9. doi: 10.1016/j.foodchem.2014.02.160. Epub 2014 Mar 12.
(1)H NMR spectroscopy was used to investigate changes in the low molecular weight metabolic profile of raw mullet (Mugil spp.) roes during frozen storage and upon processing. NMR data were analysed by Principal Component Analyses (PCA). In the model constructed using frozen roes, no statistical significant metabolic modifications were observed in the first six months of storage, while choline derivatives, dimethylamine, lactate, and most of the free amino acids were identified as changing with statistical significance (p<0.05) in response to frozen storage time of twelve months. The PCA model comparing the metabolic profiles of roes before and after processing showed that the major modifications occurring upon manufacturing were the increase of the choline derivative compounds, uracil, and free amino acids, and a large decrease of taurine, glucose, lactate, and creatine/phosphocreatine. All of the above mentioned modifications reflect the occurrence of chemical/biochemical reactions arising from degradation processes such as lipolysis and proteolysis.
采用¹H NMR光谱法研究了鲻鱼（鲻属）鱼籽在冷冻储存和加工过程中低分子量代谢谱的变化。通过主成分分析（PCA）对NMR数据进行分析。在使用冷冻鱼籽构建的模型中，储存的前六个月未观察到统计学上显著的代谢变化，而在储存十二个月后，胆碱衍生物、二甲胺、乳酸以及大部分游离氨基酸被确定为有统计学意义的变化（p<0.05）。比较加工前后鱼籽代谢谱的PCA模型表明，加工过程中发生的主要变化是胆碱衍生物化合物、尿嘧啶和游离氨基酸增加，以及牛磺酸、葡萄糖、乳酸和肌酸/磷酸肌酸大幅减少。上述所有变化反映了脂解和蛋白水解等降解过程引发的化学/生化反应的发生。
