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一项基于气相色谱-质谱联用技术的商业马铃薯品种块茎贮藏期代谢组学研究。

A GC-MS-based metabolomics study on the tubers of commercial potato cultivars upon storage.

作者信息

Uri Csilla, Juhász Zsófia, Polgár Zsolt, Bánfalvi Zsófia

机构信息

Agricultural Biotechnology Center, P.O. Box 411, H-2101 Gödöllő, Hungary.

University of Pannonia, Centre of Agricultural Sciences, Potato Research Centre, P.O. Box 71, H-8361 Keszthely, Hungary.

出版信息

Food Chem. 2014 Sep 15;159:287-92. doi: 10.1016/j.foodchem.2014.03.010. Epub 2014 Mar 14.

Abstract

Using gas chromatography-mass spectrometry (GC-MS) as a system for the detection of amino acids, organic acids, sugars, sugar alcohols, and fatty acids, we characterised six commercial potato cultivars (Hópehely, Katica, Lorett, Somogyi kifli, Vénusz Gold, and White Lady) with different pedigrees, starch contents, cooking types, and dormancy periods, in five developmental stages from harvest to sprouting. The tubers were stored at 20-22°C in the dark. The metabolite data were subjected to principal component analysis. No correlation between metabolite contents of freshly harvested tubers and starch content or cooking type of the cultivars was detected. The storage decreased the fructose and sucrose and increased the proline concentrations of tubers. Irrespective of the length of dormancy a substantial difference in metabolite composition at each time point upon storage was detected in each cultivar except Somogyi kifli, the only cultivar amongst those tested with a pure Solanum tuberosum origin and A cooking type.

摘要

我们使用气相色谱 - 质谱联用仪(GC - MS)作为检测氨基酸、有机酸、糖类、糖醇类和脂肪酸的系统,对六个具有不同谱系、淀粉含量、烹饪类型和休眠期的商业马铃薯品种(霍佩赫利、卡蒂察、洛雷、绍莫吉吉夫利、维纳斯黄金和白夫人)在从收获到发芽的五个发育阶段进行了表征。块茎在黑暗中于20 - 22°C下储存。对代谢物数据进行了主成分分析。未检测到刚收获块茎的代谢物含量与品种的淀粉含量或烹饪类型之间存在相关性。储存使块茎中的果糖和蔗糖含量降低,脯氨酸浓度升高。除绍莫吉吉夫利外,在每个品种储存后的每个时间点,无论休眠期长短,均检测到代谢物组成存在显著差异,绍莫吉吉夫利是所测试品种中唯一具有纯马铃薯起源且为A烹饪类型的品种。

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