Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK.
J Agric Food Chem. 2010 Jan 27;58(2):1214-23. doi: 10.1021/jf903104b.
Phytochemical diversity was examined by gas chromatography-mass spectrometry in tubers of genotypes belonging to groups Andigena, Phureja, Stenotomum, and Tuberosum of the potato, Solanum tuberosum. Polar extracts (mainly amino acids, organic acids, sugars, and sugar alcohols) and nonpolar extracts (mainly fatty acids, fatty alcohols, and sterols) were examined. There was a large range in levels of metabolites, including those such as asparagine, fructose, and glucose, that are important to tuber quality, offering considerable scope for selecting germplasm for breeding programmes. There were significant differences in the levels of many metabolites among the groups. The metabolite profiles of genotypes belonging to Phureja and Stenotomum were similar and different from those of Tuberosum and the majority of Andigena genotypes. There was some agreement with the phylogeny of the groups in that Stenotomum is believed to be the ancestor of Phureja and they are both distinct from Tuberosum. Andigena genotypes could be partially distinguished according to geographical origin, Bolivian genotypes being particularly distinct from those from Ecuador. Biosynthetic links between metabolites were explored by performing pairwise correlations of all metabolites. The significance of some expected and unexpected strong correlations between many amino acids (e.g., between isoleucine, lysine, valine, and other amino acids) and between several nonpolar metabolites (e.g., between many fatty acids) is discussed. For polar metabolites, correlation analysis gave essentially similar results irrespective of whether the whole data set, only Andigena genotypes, or only Phureja genotypes were used. In contrast, for the nonpolar metabolites, Andigena only and Phureja only data sets resulted in weaker and stronger correlations, respectively, compared to the whole data set, and may suggest differences in the biochemistry of the two groups, although the interpretation should be viewed with some caution.
采用气相色谱-质谱法对属于马铃薯（Solanum tuberosum）Andigena、Phureja、stenotomum 和 Tuberosum 组的基因型块茎中的植物化学物质多样性进行了研究。考察了极性提取物（主要为氨基酸、有机酸、糖和糖醇）和非极性提取物（主要为脂肪酸、脂肪醇和甾醇）。代谢物水平差异很大，包括天冬酰胺、果糖和葡萄糖等对块茎质量很重要的物质，这为选择种质进行育种计划提供了很大的空间。各组之间许多代谢物的水平存在显著差异。属于 Phureja 和 stenotomum 的基因型的代谢物图谱相似，与 Tuberosum 和大多数 Andigena 基因型的代谢物图谱不同。这与各组的系统发育有些一致，因为 stenotomum 被认为是 Phureja 的祖先，它们都与 Tuberosum 不同。根据地理起源，Andigena 基因型可以部分区分，玻利维亚基因型与厄瓜多尔的基因型特别不同。通过对所有代谢物进行成对相关分析，探讨了代谢物之间的生物合成联系。讨论了一些预期和意外的强相关性（例如，异亮氨酸、赖氨酸、缬氨酸和其他氨基酸之间，以及几种非极性代谢物之间）的意义。对于极性代谢物，无论使用整个数据集、仅 Andigena 基因型还是仅 Phureja 基因型，相关分析的结果基本相同。相比之下，对于非极性代谢物，与整个数据集相比，Andigena 仅和 Phureja 仅数据集分别导致相关性较弱和较强，这可能表明两组之间的生物化学存在差异，尽管应谨慎解释。
