Chantarasataporn Patomporn, Tepkasikul Preenapha, Kingcha Yutthana, Yoksan Rangrong, Pichyangkura Rath, Visessanguan Wonnop, Chirachanchai Suwabun
The Petroleum and Petrochemical College, Chulalongkorn University, Soi Chula 12, Phyathai Rd., Pathumwan, Bangkok 10330, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Rd., Klong Nueng, Klong Luang, Pathumthani 12120, Thailand.
Food Chem. 2014 Sep 15;159:463-70. doi: 10.1016/j.foodchem.2014.03.019. Epub 2014 Mar 13.
Water-based chitosans in the forms of oligochitosan (OligoCS) and nanowhisker chitosan (CSWK) are proposed as a novel food preservative based on a minced pork model study. The high surface area with a positive charge over the neutral pH range (pH 5-8) of OligoCS and CSWK lead to an inhibition against Gram-positive (Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus) and Gram-negative microbes (Salmonella enteritidis and Escherichia coli O157:H7). In the minced pork model, OligoCS effectively performs a food preservative for shelf-life extension as clarified from the retardation of microbial growth, biogenic amine formation and lipid oxidation during the storage. OligoCS maintains almost all myosin heavy chain protein degradation as observed in the electrophoresis. The present work points out that water-based chitosan with its unique morphology not only significantly inhibits antimicrobial activity but also maintains the meat quality with an extension of shelf-life, and thus has the potential to be used as a food preservative.
基于猪肉糜模型研究,提出将低聚壳聚糖(OligoCS)和纳米晶须壳聚糖(CSWK)形式的水性壳聚糖作为一种新型食品防腐剂。OligoCS和CSWK在中性pH范围(pH 5 - 8)内具有高表面积和正电荷,导致对革兰氏阳性菌(金黄色葡萄球菌、单核细胞增生李斯特菌和蜡样芽孢杆菌)和革兰氏阴性菌(肠炎沙门氏菌和大肠杆菌O157:H7)有抑制作用。在猪肉糜模型中,从储存期间微生物生长、生物胺形成和脂质氧化的延缓情况可以看出,OligoCS有效地起到了延长保质期的食品防腐剂作用。如电泳观察所示,OligoCS几乎能维持所有肌球蛋白重链蛋白的降解。目前的研究指出,具有独特形态的水性壳聚糖不仅能显著抑制抗菌活性,还能通过延长保质期来保持肉质,因此有潜力用作食品防腐剂。