Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
Department of Civil and Environmental Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Bioresour Technol. 2014 Aug;165:158-61. doi: 10.1016/j.biortech.2014.03.097. Epub 2014 Mar 27.
In the present work, lactic acid (LA) production performance with granulation was investigated at various hydraulic retention times (HRTs), 8-0.5h. Glucose was used as a feedstock, and anaerobic mixed cultures were inoculated in an up-flow anaerobic sludge blanket reactor. As HRT decreased, the average diameter and hydrophobicity of the granules increased from 0.31 to 3.4mm and from 17.5% to 38.3%, respectively, suggesting the successful formation of granules. With decreasing HRT, LA productivity increased up to 16.7gLA/L-fermenter/h at HRT 0.5h. The existence of rod-shaped organisms with pores and internal channels at granule surface was observed by scanning electron microscope. Next generation sequencing revealed that Lactobacillus was the dominant microorganism, accounting for 96.7% of total sequences, comprising LA-producing granules.
在本工作中，研究了不同水力停留时间（HRT），8-0.5h 下的颗粒状乳酸（LA）生产性能。以葡萄糖为原料，在升流式厌氧污泥床反应器中接种厌氧混合培养物。随着 HRT 的降低，颗粒的平均直径和疏水性从 0.31 增加到 3.4mm 和从 17.5%增加到 38.3%，表明颗粒成功形成。随着 HRT 的降低，LA 生产力在 HRT 为 0.5h 时增加到 16.7gLA/L-发酵罐/h。通过扫描电子显微镜观察到颗粒表面存在带孔和内部通道的杆状生物。下一代测序表明，乳杆菌是优势微生物，占总序列的 96.7%，包括产 LA 的颗粒。
