Apparicio Maricy, Mostachio Giuliano Q, Motheo Tathiana F, Alves Aracelle E, Padilha Luciana, Pires-Butler Eliandra A, Savi Paula A P, Uscategui Ricardo A R, Luvoni Gaia C, Vicente Wilter R R
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Università degli Studi di Milano, Via Celoria 10, 20133 Milano, Italy.
Reprod Fertil Dev. 2015 Sep;27(7):1082-7. doi: 10.1071/RD14022.
The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72h in base medium (BM) with 10IUmL(-1) human chorionic gonadotrophin (hCG), 1μgmL(-1) progesterone (P4) and 1μgmL(-1) oestradiol (E2); in bi-phasic System B oocytes were matured for 48h in BM with hCG and for 24h in BM with P4; in bi-phasic System C oocytes were matured for 48h in BM with hCG, P4 and E2, and for 24h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (P<0.05). Bi-phasic systems were also beneficial for cortical granule distribution (an indication of cytoplasmic maturation) and its relationship to nuclear status: 74.5% of the oocytes cultured in System B and 85.4% of those cultured in System C presented both nuclear and cytoplasmic maturation (P<0.001). The stage of the oestrous cycle did not influence maturation rates.
