Department of Animal Health, Faculty of Veterinary Sciences, Complutense University, 28040 Madrid, Spain.
Department of Internal Medicine and Animal Surgery, Faculty of Veterinary Sciences, Complutense University, 28040 Madrid, Spain; Animal Health Surveillance Center (VISAVET), Complutense University of Madrid, 28040 Madrid, Spain.
Vet Microbiol. 2014 Jun 25;171(1-2):248-54. doi: 10.1016/j.vetmic.2014.03.029. Epub 2014 Mar 30.
Zebrafish has been used for studying infections and host-pathogen interactions in different bacterial fish pathogens. In the present study we evaluated the ability of Lactococcus garvieae to infect zebrafish when inoculated intraperitoneally with 2 × 10(7)UFC of this pathogen. L. garvieae can colonize and invade zebrafish at multiple anatomical sites causing a lethal acute septicemic infection with clinical signs and lesions consistent with those observed in lactococcosis outbreaks. Immunohistochemical studies showed the presence of L. garvieae into macrophages as well as into non-phagocytic zebrafish cells of liver (hepatocytes). The internalization capacity showed by L. garvieae in zebrafish cells was confirmed in the rainbow trout cell line RTG-2. Our results provide the first evidence that L. garvieae is able to invade non-phagocytic host cells.
斑马鱼已被用于研究不同鱼类细菌病原体的感染和宿主-病原体相互作用。在本研究中，我们评估了 2×10(7)UFC 这种病原体经腹腔接种感染斑马鱼的能力。L. garvieae 可以在多个解剖部位定殖和侵袭斑马鱼，导致具有临床症状和病变的致死性急性败血性感染，与乳球菌病暴发中观察到的一致。免疫组织化学研究表明，L. garvieae 存在于巨噬细胞以及肝脏（肝细胞）的非吞噬性斑马鱼细胞中。在虹鳟鱼细胞系 RTG-2 中证实了 L. garvieae 在斑马鱼细胞中的内化能力。我们的研究结果首次提供了证据表明 L. garvieae 能够侵袭非吞噬性宿主细胞。
