Department of Zoology, University of Pune, Ganeshkhind Road, Pune 411007, India.
Laboratory of Integrative Neuroendocrinology, Department of Veterinary Biomedical Sciences, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
Gen Comp Endocrinol. 2014 Jun 1;202:8-14. doi: 10.1016/j.ygcen.2014.04.007. Epub 2014 Apr 24.
Nesfatin-1 is a recently discovered anorectic protein derived from the precursor nucleobindin-2 (NUCB2). While nesfatin-1 has been widely studied in mammals, and goldfish, there are no reports of nesfatin-1 in amphibians. Using immunohistochemistry and Western blot analysis, this study assessed the distribution of NUCB2/nesfatin-1 in the brain of frog Microhyla ornata. NUCB2/nesfatin-1 like immunoreactivity was found in the olfactory receptor neurons, Bowman's glands and in the olfactory epithelium of medial diverticulum. In the brain, immunoreactive perikarya were seen in the anterior preoptic area, magnocellular nucleus, suprachiasmatic nucleus, ventromedial thalamic nucleus, posterior thalamic nucleus, nucleus infundibularis ventralis and dorsalis, and the cerebellar nucleus. NUCB2/nesfatin-1like immunoreactivity was also detected in the pineal and pituitary glands of frog. The presence of NUCB2/nesfatin-1 in the key brain regions suggest possible roles for this protein in the regulation of physiological processes in frogs.
nesfatin-1 是一种新发现的来自前体核蛋白 2（NUCB2）的厌食蛋白。虽然 nesfatin-1 在哺乳动物和金鱼中被广泛研究，但在两栖动物中尚无 nesfatin-1 的报道。本研究通过免疫组织化学和 Western blot 分析评估了蛙 Microhyla ornata 脑中 NUCB2/nesfatin-1 的分布。发现 NUCB2/nesfatin-1 样免疫反应性存在于嗅觉受体神经元、鲍曼氏腺和中鼻道嗅上皮中。在脑中，在前视前区、大细胞核、视交叉上核、腹内侧丘脑核、后丘脑核、下丘脑腹侧和背核以及小脑核中可见免疫活性胞体。在蛙的松果腺和垂体中也检测到了 NUCB2/nesfatin-1 样免疫反应性。NUCB2/nesfatin-1 在前脑关键区域的存在表明该蛋白可能在调节青蛙的生理过程中发挥作用。
