Department of Organismal Animal Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
Department of Organismal Animal Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
Gen Comp Endocrinol. 2014 Jun 1;202:69-75. doi: 10.1016/j.ygcen.2014.04.010. Epub 2014 Apr 24.
Corticotropin-releasing factor-binding protein (CRF-BP) is considered a key determinant for CRF receptor (CRF-R) activation by CRF and several related peptides. Earlier studies have shown that the CRF system is highly conserved in gene structures throughout evolution, yet little is known about the evolutionary conservation of its biological functions. Therefore, we address the functional properties of CRF-BP and CRF-Rs in a teleost fish (common carp; Cyprinus carpio L.). We report the finding of two similar, yet distinct, genes for both CRF-R1 and CRF-R2 in this species. The four receptors are differentially responsive to CRF, urotensin-I (UI), sauvagine, and urocortin-2 (Ucn-2) and -3 (Ucn-3) as shown by luciferase assays. In vitro, carp CRF-BP inhibits CRF- and UI-mediated activation of the newfound CRF-Rs, but its potency to do so varies between receptor and peptide ligand. This is the first paper to establish the functionality and physiological interplay between CRF-BP, CRF-Rs and CRF-family peptides in a teleostean species.
促肾上腺皮质素释放因子结合蛋白（CRF-BP）被认为是 CRF 和几种相关肽激活 CRF 受体（CRF-R）的关键决定因素。早期的研究表明，CRF 系统在进化过程中的基因结构中高度保守，但对其生物学功能的进化保守性知之甚少。因此，我们在一种硬骨鱼（鲤鱼；Cyprinus carpio L.）中研究了 CRF-BP 和 CRF-Rs 的功能特性。我们在该物种中发现了两种类似但又不同的 CRF-R1 和 CRF-R2 基因。荧光素酶测定显示，这四种受体对 CRF、urotensin-I (UI)、sauvagine 和 urocortin-2 (Ucn-2) 和 -3 (Ucn-3) 的反应不同。在体外，鲤鱼 CRF-BP 抑制新发现的 CRF-Rs 对 CRF 和 UI 的激活，但它抑制的效力因受体和肽配体而异。这是第一篇在硬骨鱼类中建立 CRF-BP、CRF-Rs 和 CRF 家族肽之间功能和生理相互作用的论文。
