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野生枸杞叶提取物作为发酵干香肠中亚硝酸盐替代物的抗氧化和抗菌活性。

Antioxidant and antimicrobial activity of Kitaibelia vitifolia extract as alternative to the added nitrite in fermented dry sausage.

机构信息

Department of Food Technology, Faculty of Agronomy, University of Kragujevac, Cara Dušana 34, 32000 Čačak, Republic of Serbia.

Department of Chemistry and Chemical Engineering, Faculty of Agronomy, University of Kragujevac, Cara Dušana 34, 32000 Čačak, Republic of Serbia.

出版信息

Meat Sci. 2014 Aug;97(4):459-67. doi: 10.1016/j.meatsci.2014.03.012. Epub 2014 Mar 26.

Abstract

Fermented dry sausages (FDS) without nitrite added, fortified with bioactive phenol and flavonoid compounds originating from the ethanol extract of Kitaibelia vitifolia were food matrix for investigation of its antioxidant and antimicrobial potency. These activities were researched in order to improve the sausages' shelf-life, safety, and provide health benefits to consumers as well. The oxidative stability of the FDS, containing two different levels of natural preservative, was evaluated using five different contemporary methods for antioxidative activity. The activity was tested on the 20th day of the refrigerated storage. Minimum inhibitory concentrations of the sausage extract were determined against six microorganisms, using a micro dilution method. Determined optimal effective concentration of dissolved K. vitifolia extract (12.5 g/kg of meat dough) revealed strong antioxidant activity, and moderate antimicrobial activity against Escherichia coli (minimum inhibitory concentrations=15.625 μg/mL). The modified sausages had typical chemical-physical characteristics of FDS, controlled on 0, 13, 26 d of ripening and 20, 40 and 60 d of storage.

摘要

未添加亚硝酸盐的发酵干香肠(FDS),添加了来自华西忍冬乙醇提取物的生物活性酚类和类黄酮化合物,作为研究其抗氧化和抗菌效力的食品基质。这些活动旨在提高香肠的保质期、安全性,并为消费者提供健康益处。使用五种不同的当代抗氧化活性方法评估了含有两种不同天然防腐剂水平的 FDS 的氧化稳定性。在冷藏储存的第 20 天测试了活性。使用微量稀释法测定了香肠提取物对六种微生物的最小抑菌浓度。确定了溶解的华西忍冬提取物的最佳有效浓度(12.5 g/kg 肉馅),显示出很强的抗氧化活性和对大肠杆菌的适度抗菌活性(最小抑菌浓度=15.625 μg/mL)。改良香肠具有 FDS 的典型理化特性,在成熟 0、13、26 天和储存 20、40 和 60 天进行了控制。

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