Faculty of Agronomy, University of Kragujevac, Čačak, Serbia.
J Med Food. 2011 Dec;14(12):1617-23. doi: 10.1089/jmf.2011.0013. Epub 2011 Aug 30.
This study was aimed at evaluating the antioxidant activity and efficacy of the ethanolic extract of the endemic plant species Kitaibelia vitifolia in inhibiting the growth of selected fungi and bacteria. Antimicrobial activity was tested using the broth dilution procedure for determination of minimum inhibitory concentration (MIC). MICs were determined for eight selected indicator strains. The highest susceptibility to K. vitifolia ethanolic extract among the bacteria tested was exhibited by Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, and Klebsiella pneumoniae ATCC 13883 (MIC=15.62 μg/mL), followed by Escherichia coli ATCC 25922 and Proteus mirabilis ATCC 14153 (MIC=31.25 μg/mL), and Proteus vulgaris ATCC 13315 (MIC=62.50 μg/mL). Of the fungi, Candida albicans ATCC 10231 (MIC=15.62 μg/mL) showed the highest susceptibility, and Aspergillus niger ATCC 16404 (MIC=31.25 μg/mL) had the lowest. Results showed that K. vitifolia extract possesses antioxidant activity, with total antioxidant capacity of 75.45±0.68 μg of ascorbic acid/g and 50% inhibition concentration values of 47.45±0.55 μg/mL for 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, 35.35±0.68 μg/mL for inhibitory activity against lipid peroxidation, 95.25±0.52 μg/mL for hydroxyl radical scavenging activity, and 31.50±0.35 μg/mL for metal chelating activity. Total phenolics, flavonoids, condensed tannins, and gallotannins were 85.25±0.69 mg of gallic acid (GA)/g, 45.32±0.55 mg of rutin/g, 54.25±0.75 mg of GA/g, and 41.74±0.55 mg of GA/g, respectively. The phenolic composition of K. vitifolia extract was determined by high-performance liquid chromatography. Rosmarinic acid was found to be the dominant phenolic compound of the extract.
本研究旨在评估地方性植物物种 Kitaibelia vitifolia 的乙醇提取物的抗氧化活性和功效，以抑制所选真菌和细菌的生长。使用肉汤稀释程序测试抗菌活性以确定最小抑菌浓度（MIC）。对八种选定的指示菌株进行 MIC 测定。在所测试的细菌中，对 Kitaibelia vitifolia 乙醇提取物表现出最高敏感性的是枯草芽孢杆菌 ATCC 6633、金黄色葡萄球菌 ATCC 25923 和肺炎克雷伯菌 ATCC 13883（MIC=15.62μg/mL），其次是大肠杆菌 ATCC 25922 和奇异变形杆菌 ATCC 14153（MIC=31.25μg/mL）和普通变形杆菌 ATCC 13315（MIC=62.50μg/mL）。在真菌中，白色念珠菌 ATCC 10231（MIC=15.62μg/mL）表现出最高的敏感性，黑曲霉 ATCC 16404（MIC=31.25μg/mL）的敏感性最低。结果表明，Kitaibelia vitifolia 提取物具有抗氧化活性，其总抗氧化能力为 75.45±0.68μg 抗坏血酸/g，2,2-二苯基-1-苦基肼自由基清除活性的 50%抑制浓度值为 47.45±0.55μg/mL，抑制脂质过氧化的抑制活性为 35.35±0.68μg/mL，羟基自由基清除活性为 95.25±0.52μg/mL，金属螯合活性为 31.50±0.35μg/mL。总酚、类黄酮、缩合单宁和没食子单宁分别为 85.25±0.69mg 没食子酸（GA）/g、45.32±0.55mg 芦丁/g、54.25±0.75mg GA/g 和 41.74±0.55mg GA/g。通过高效液相色谱法确定了 Kitaibelia vitifolia 提取物的酚类组成。迷迭香酸被发现是提取物中的主要酚类化合物。
