Chuderland Dana, Ben-Ami Ido, Friedler Shevach, Hasky Noa, Ninio-Many Lihi, Goldberg Keren, Bar-Joseph Hadas, Grossman Hadas, Shalgi Ruth
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.
IVF and Infertility Unit, Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Zerifin 70300, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Mol Cell Endocrinol. 2014 Jun 5;390(1-2):85-92. doi: 10.1016/j.mce.2014.04.006. Epub 2014 Apr 24.
Pigment epithelium-derived factor (PEDF) is highly expressed in the female reproductive system and is subjected to regulation by steroid hormones in the ovary. As the uterine endometrium exhibits morphological and functional changes in response to estrogen (E2) and progesterone (P4), we aimed at characterizing the expression of PEDF in this component of the female reproductive tract and further at exploring the hormonal regulation of its expression. We found that PEDF is expressed in human and mouse endometrium. We further showed that this expression is subjected to regulation by steroid hormones, both in vivo and in vitro, as follows: E2 decreased PEDF expression and P4 increased its levels. In human endometrial samples, PEDF levels were dynamically altered along the menstrual cycle; they were low at the proliferative and early secretory phases and significantly higher at the late secretory phase. The expression levels of PEDF were inversely correlated to that of vascular endothelial growth factor (VEGF). We also showed that PEDF receptor was expressed in the endometrium and that its stimulation reduced VEGF expression. Illustrating the pattern of PEDF expression during the menstrual cycle may contribute to our understanding of the endometrial complexity.
色素上皮衍生因子（PEDF）在女性生殖系统中高度表达，并在卵巢中受到甾体激素的调节。由于子宫内膜会因雌激素（E2）和孕激素（P4）而出现形态和功能变化，我们旨在确定PEDF在女性生殖道这一组成部分中的表达特征，并进一步探索其表达的激素调节机制。我们发现PEDF在人和小鼠的子宫内膜中均有表达。我们进一步表明，这种表达在体内和体外均受甾体激素调节，具体如下：E2降低PEDF表达，P4升高其水平。在人类子宫内膜样本中，PEDF水平在月经周期中动态变化；在增殖期和分泌早期较低，在分泌晚期显著升高。PEDF的表达水平与血管内皮生长因子（VEGF）的表达水平呈负相关。我们还表明，PEDF受体在子宫内膜中表达，其刺激可降低VEGF表达。阐明月经周期中PEDF的表达模式可能有助于我们理解子宫内膜的复杂性。
