Church William H, Adams Ryan E, Wyss Livia S
Department of Chemistry and Neuroscience Program, Trinity College, Hartford, CT, USA.
Department of Chemistry and Neuroscience Program, Trinity College, Hartford, CT, USA.
Neurosci Lett. 2014 Jun 13;571:1-4. doi: 10.1016/j.neulet.2014.04.016. Epub 2014 Apr 24.
The present study was conducted to determine if the ketogenic diet altered basal levels of monoamine neurotransmitters in mice. The catecholamines dopamine (DA) and norephinephrine (NE) and the indolamine serotonin (5HT) were quantified postmortem in six different brain regions of adult mice fed a ketogenic diet for 3 weeks. The dopamine metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) and the serotonin metabolite 5-hydroxyindole acetic acid (5HIAA) were also measured. Tissue punches were collected bilaterally from the motor cortex, somatosensory cortex, nucleus accumbens, anterior caudate-putamen, posterior caudate-putamen and the midbrain. Dopaminergic activity, as measured by the dopamine metabolites to dopamine content ratio - ([DOPAC]+[HVA])/[DA] - was significantly increased in the motor and somatosensory cortex regions of mice fed the ketogenic diet when compared to those same areas in brains of mice fed a normal diet. These results indicate that the ketogenic diet alters the activity of the meso-cortical dopaminergic system, which may contribute to the diet's therapeutic effect in reducing epileptic seizure activity.
本研究旨在确定生酮饮食是否会改变小鼠单胺类神经递质的基础水平。对喂食生酮饮食3周的成年小鼠的六个不同脑区进行死后分析，以定量儿茶酚胺多巴胺（DA）、去甲肾上腺素（NE）和吲哚胺5-羟色胺（5HT）。还测定了多巴胺代谢产物3,4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）以及5-羟色胺代谢产物5-羟吲哚乙酸（5HIAA）。从运动皮层、体感皮层、伏隔核、前尾状核-壳核、后尾状核-壳核和中脑双侧采集组织块。与喂食正常饮食的小鼠脑内相同区域相比，喂食生酮饮食的小鼠运动和体感皮层区域中，以多巴胺代谢产物与多巴胺含量之比 - （[DOPAC]+[HVA]）/[DA] - 衡量的多巴胺能活性显著增加。这些结果表明，生酮饮食改变了中脑-皮层多巴胺能系统的活性，这可能有助于该饮食在降低癫痫发作活动方面的治疗效果。
