Almaida-Pagán Pedro F, Lucas-Sánchez Alejandro, Tocher Douglas R
Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, United Kingdom.
Department of Physiology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.
Biochim Biophys Acta. 2014 Jul;1841(7):1003-11. doi: 10.1016/j.bbalip.2014.04.004. Epub 2014 Apr 24.
Considering membranes and membrane components as possible pacemakers of the main processes taking place inside mitochondria, changes in phospholipids or fatty acids could play a central role linking different mechanisms involved in cumulative damage to cell molecules and dysfunction during periods of high stress, such as rapid growth and aging. Changes affecting either lipid class or fatty acid compositions could affect phospholipid and membrane properties and alter mitochondrial function and cell viability. In the present study, mitochondrial oxidative status and mitochondrial membrane phospholipid compositions were analyzed throughout the life-cycle of zebrafish. TBARS content significantly increased in 18-month-old fish while aconitase activity decreased in 24-month-old fish, which have been related with oxidative damage to molecules. Mitochondria-specific superoxide dismutase decreased in 24-month-old animals although this change was not statistically significant. Age affected both mitochondrial phospholipid content and the peroxidation index of most phospholipid classes suggesting that oxidative damage to mitochondrial lipids was occurring.
考虑到膜和膜成分可能是线粒体内部主要过程的潜在起搏器，磷脂或脂肪酸的变化可能在连接高压力时期（如快速生长和衰老）细胞分子累积损伤和功能障碍所涉及的不同机制中发挥核心作用。影响脂质类别或脂肪酸组成的变化可能会影响磷脂和膜的性质，并改变线粒体功能和细胞活力。在本研究中，对斑马鱼整个生命周期的线粒体氧化状态和线粒体膜磷脂组成进行了分析。18个月大的鱼中硫代巴比妥酸反应物（TBARS）含量显著增加，而24个月大的鱼中乌头酸酶活性下降，这与分子的氧化损伤有关。24个月大的动物中线粒体特异性超氧化物歧化酶减少，尽管这一变化在统计学上不显著。年龄影响线粒体磷脂含量以及大多数磷脂类别的过氧化指数，表明线粒体脂质正在发生氧化损伤。
