Clemente Moreno Maria José, Hevin Cyril, Ollat Nathalie, Cookson Sarah Jane
ISVV; UMR 1287 EGFV; Villenave d'Ornon; Aquitaine, France.
Plant Signal Behav. 2014 Apr 25;9. doi: 10.4161/psb.28852.
Gene expression changes induced during graft union formation (the first month after grafting) in grapevine have been studied using whole genome microarrays. The genes differentially expressed between the rootstock and graft interface tissues of homo-grafts (Cabernet Sauvignon (CS) grafted onto CS) were compared at 3 and 28 days after grafting (dag). Graft union formation was associated with the upregulation of genes involved in secondary metabolism, cell wall, wound responses and hormone signaling. These gene expression differences were associated with the accumulation of lignin, cellulose and callose in the callus cells. Superimposed upon this, hetero-grafting between two different grapevine genotypes resulted in the further upregulation of stress and/or defense responses at the graft interface. Here we discuss the limitations of the techniques used to study the developments at the graft interface to date and future research directions to understand graft union formation in plants.
利用全基因组微阵列研究了葡萄嫁接结合部形成过程（嫁接后第一个月）中诱导的基因表达变化。比较了同品种嫁接（赤霞珠嫁接到赤霞珠）在嫁接后3天和28天砧木与嫁接结合部组织之间差异表达的基因。嫁接结合部的形成与参与次生代谢、细胞壁、伤口反应和激素信号传导的基因上调有关。这些基因表达差异与愈伤组织细胞中木质素、纤维素和胼胝质的积累有关。在此基础上，两种不同葡萄基因型之间的异源嫁接导致嫁接结合部应激和/或防御反应的进一步上调。本文讨论了迄今为止用于研究嫁接结合部发育的技术的局限性以及未来理解植物嫁接结合部形成的研究方向。
