Opoku-Agyemang Frank, Amissah Jacqueline Naalamle, Owusu-Nketia Stella, Ofori Peter Amoako, Notaguchi Michitaka
College of Basic and Applied Sciences (CBAS), University of Ghana (UG), P.O. Box LG 25, Legon, Accra, Ghana.
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya 464-8601, Japan.
MethodsX. 2024 Aug 19;13:102904. doi: 10.1016/j.mex.2024.102904. eCollection 2024 Dec.
Grafting techniques have been successfully adopted to improve resistance to biotic and abiotic stresses, increase yields, fruit quality and study systemic signaling in plants. This technique has not been fully explored in cassava and there is currently no standardized grafting method for this species published especially in Africa. This is the first report on cassava grafting protocol in Africa with valuable advantages including utilizing a cost-effective and environmentally friendly wooden healing chamber. In this study, we describe an optimized cleft grafting protocol for cassava utilizing a wooden healing chamber and outline the step-by-step procedure with optimum conditions to generate a high grafting success rate. Using a top wedge grafting technique with high reproducibility and success rates, we developed a straightforward and robust grafting protocol for cassava () cultivars. Grafting success was recorded and this protocol produced a high grafting success of 90 % and its reproducibility makes it suitable for mass production thereby addressing the need for efficient cassava propagation. This grafting protocol requires less specialized equipment and expertise making it more accessible to farmers and researchers with limited resources to promote the use of grafting for cassava growth, yield improvement and advanced studies such as systemic long-distance signaling in plants.•Optimization of cleft grafting method obtains a high success grafting rate of cassava.•A wooden healing chamber provides a controlled environment for graft healing.•Promoting cassava grafting; a priority to produce new cultivars and explore breeding research prospects.
嫁接技术已成功应用于提高植物对生物和非生物胁迫的抗性、增加产量、改善果实品质以及研究植物的系统信号传导。该技术在木薯中尚未得到充分探索，目前尤其在非洲，还没有针对该物种发布的标准化嫁接方法。这是非洲关于木薯嫁接方案的首份报告，具有诸多宝贵优势，包括使用了经济高效且环保的木质愈合室。在本研究中，我们描述了一种利用木质愈合室对木薯进行优化的劈接方案，并概述了在最佳条件下实现高嫁接成功率的详细步骤。通过使用具有高重现性和成功率的顶楔嫁接技术，我们为木薯（）品种开发了一种简单且可靠的嫁接方案。记录了嫁接成功率，该方案产生了90%的高嫁接成功率，其重现性使其适用于大规模生产，从而满足了木薯高效繁殖的需求。这种嫁接方案所需的专业设备和专业知识较少，使资源有限的农民和研究人员更容易采用，以促进嫁接技术在木薯生长、产量提高以及植物系统长距离信号传导等高级研究中的应用。•劈接方法的优化获得了木薯高成功率的嫁接。•木质愈合室为嫁接愈合提供了可控环境。•促进木薯嫁接；是培育新品种和探索育种研究前景的优先事项。
