Melander T, Köhler C, Nilsson S, Hökfelt T, Brodin E, Theodorsson E, Bartfai T
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
J Chem Neuroanat. 1988 Jul-Aug;1(4):213-33.
The distribution of 125I-galanin binding sites in the rat central nervous system was studied by the use of autoradiography, and the amount of peptide bound was determined by the use of microdensitometry. The amount of 125I-galanin bound in various CNS regions ranged from non detectable to 0.40 pmol per gramme tissue at a concentration of 1.5 nM 125I-galanin. The anatomical mapping revealed high density of binding sites in the telencephalon, where labelling was seen in the entorhinal, perirhinal and piriform cortices, in most septal nuclei, in the amygdala and in the hippocampal formation. In the diencephalon binding sites were densely accumulated in the hypothalamus, including the median eminence and periventricular nucleus as well as in the dorsal and medial thalamic nuclei. In the mesencephalon binding sites were seen in many nuclei including the dorsal raphe nucleus, the periaqueductal central grey and pars compacta of the substantia nigra. The pons/medulla revealed high density of binding sites in the locus coeruleus, parabrachial nuclei and in the dorsal vagal complex, while binding in the spinal cord was seen in laminae I, II and X as well as in the intermediolateral horn. The distribution of 125I-galanin binding sites corresponded well to the localization of the peptide as revealed in several earlier immunohistochemical and radioimmunoassay studies, as well as to several previously reported effects of galanin on central autonomic functions.
采用放射自显影术研究了大鼠中枢神经系统中125I-甘丙肽结合位点的分布，并运用显微密度测定法测定了结合的肽量。在1.5 nM 125I-甘丙肽浓度下，各中枢神经系统区域中结合的125I-甘丙肽量在每克组织不可检测至0.40 pmol之间。解剖定位显示，端脑中结合位点密度较高，在内嗅皮质、梨状前皮质和梨状皮质、大多数隔核、杏仁核和海马结构中可见标记。在间脑中，结合位点密集聚集在下丘脑，包括正中隆起和室旁核以及背侧和内侧丘脑核。在中脑中，在许多核中可见结合位点，包括中缝背核、导水管周围中央灰质和黑质致密部。脑桥/延髓在蓝斑、臂旁核和迷走神经背侧复合体中显示出高密度的结合位点，而在脊髓中，在I层、II层和X层以及中间外侧角可见结合。125I-甘丙肽结合位点的分布与先前一些免疫组织化学和放射免疫分析研究所揭示的肽的定位以及先前报道的甘丙肽对中枢自主功能的几种作用非常吻合。
