Cumming D A, Hellerqvist C G, Harris-Brandts M, Michnick S W, Carver J P, Bendiak B
Department of Medical Genetics, University of Toronto, Ontario, Canada.
Biochemistry. 1989 Jul 25;28(15):6500-12. doi: 10.1021/bi00441a051.
In the accompanying paper (Bendiak et al., 1989), the separation of a series of oligosaccharides released from asparagine residues of fetuin was described. A series of NMR experiments, which included one- and two-dimensional nuclear Overhauser enhancement, two-dimensional correlation spectroscopy, and two-dimensional relayed-coherence spectroscopy, as well as permethylation analyses, established a Gal beta 1----3(NeuAc alpha 2----6)GlcNAc beta 1----4Man unit common to a series of purified structures. These oligosaccharides contained either three, four, or five glycosidically linked sialic acid residues. The NeuAc residue in alpha 2----6 linkage to GlcNAc gives rise to diagnostic chemical shift perturbations of particular proton signals in the oligosaccharides.
在随附论文（本迪亚克等人，1989年）中，描述了从胎球蛋白天冬酰胺残基释放的一系列寡糖的分离过程。一系列核磁共振实验，包括一维和二维核Overhauser增强、二维相关光谱和二维接力相干光谱，以及全甲基化分析，确定了一系列纯化结构共有的Galβ1----3（NeuAcα2----6）GlcNAcβ1----4Man单元。这些寡糖含有三个、四个或五个糖苷键连接的唾液酸残基。与GlcNAc以α2----6键连接的NeuAc残基会导致寡糖中特定质子信号出现诊断性化学位移扰动。
