Nishijima Kazutoshi, Yamaguchi Shinji, Tanaka Mai, Sakai Yusuke, Koshimoto Chihiro, Morimoto Masatoshi, Watanabe Teruo, Fan Jianglin, Kitajima Shuji
Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan.
Exp Anim. 2014;63(2):149-54. doi: 10.1538/expanim.63.149.
The motility of sperm after freezing and thawing is critical for effective cryopreservation. It is known that supplementation with cholesterol-loaded cyclodextrin (CLC) improves cryosurvival of sperm in various animals. To clarify the effects of supplementation with CLC on rabbit sperm motility after freezing and thawing, rabbit sperm motility was analyzed using a computer-assisted sperm analysis system. Sperm motility with CLC supplementation was 29.4 ± 9.6% (mean ± SD), which was significantly higher than that of controls (20.8 ± 7.1%, P<0.05). The curvilinear velocity of sperm with CLC exceeded that of controls, whereas the values for linearity and wobble were significantly lower in sperm with CLC compared with controls. After artificial insemination, 44.3% of recovered ova were fertilized in the CLC-supplemented group, which was higher than the percentage in the control group (36.4%). The results indicate that supplementation with CLC improves the rate and quality of motility in rabbit sperm after freezing and thawing, and would be advantageous for successful cryopreservation.
冻融后精子的活力对于有效的冷冻保存至关重要。已知添加胆固醇负载环糊精（CLC）可提高各种动物精子的冷冻存活率。为了阐明添加CLC对兔精子冻融后活力的影响，使用计算机辅助精子分析系统对兔精子活力进行了分析。添加CLC后的精子活力为29.4±9.6%（平均值±标准差），显著高于对照组（20.8±7.1%，P<0.05）。添加CLC的精子曲线速度超过对照组，而添加CLC的精子的直线性和摆动值与对照组相比显著更低。人工授精后，添加CLC组回收的卵子中有44.3%受精，高于对照组（36.4%）。结果表明，添加CLC可提高兔精子冻融后的活力速率和质量，有利于成功冷冻保存。
