Van Dijck P, Winderickx J, Heyns W, Verhoeven G
Laboratory for Experimental Medicine and Endocrinology, Onderwijs en Navorsing, Catholic University of Leuven, Belgium.
Mol Cell Endocrinol. 1989 Jul;64(2):195-204. doi: 10.1016/0303-7207(89)90146-9.
The binding of androgen-receptor complexes to fragments derived from two alpha 2u-globulin genes (RAP 01 and RAO 01) was studied using a DNA-cellulose competition assay. Rat prostate cytosol labelled with [3H]mibolerone was used as a source of the androgen receptor. Two controls were included in these studies: the long terminal repeat (LTR) of mouse mammary tumor virus which has previously been shown to act as an androgen response element and a fragment of the C3 gene of prostatic binding protein which has been demonstrated to bind androgen-receptor complexes. Our experiments indicate that androgen-receptor complexes bind specifically and with comparable affinity to the C3 gene fragment, the LTR and a fragment of RAP 01 located in the 5'-upstream region (bp -642 up to -584). No specific interaction was observed with fragments derived from RAO 01. The region of RAP 01 which binds androgen-receptor complexes has previously been shown to interact with glucocorticoid receptors and contains a 17 bp sequence homologous with the consensus sequence for glucocorticoid-receptor binding. A mutation in this sequence in RAO 01 may be responsible for the loss of glucocorticoid and androgen-receptor binding. It is concluded that at least one member of the alpha 2u-globulin gene family interacts directly with androgen-receptor complexes with an affinity comparable to that observed for other androgen-dependent genes. The binding is observed in a region displaying also affinity for the glucocorticoid receptor.
利用DNA - 纤维素竞争分析法研究了雄激素受体复合物与源自两个α2u - 球蛋白基因（RAP 01和RAO 01）的片段的结合情况。用[3H]米勃龙标记的大鼠前列腺胞质溶胶作为雄激素受体的来源。这些研究中纳入了两个对照：先前已被证明可作为雄激素反应元件的小鼠乳腺肿瘤病毒长末端重复序列（LTR），以及已被证明能结合雄激素受体复合物的前列腺结合蛋白C3基因的一个片段。我们的实验表明，雄激素受体复合物与C3基因片段、LTR以及位于5' - 上游区域（碱基对 - 642至 - 584）的RAP 01片段特异性结合，且亲和力相当。未观察到与源自RAO 01的片段有特异性相互作用。先前已表明，RAP 01中与雄激素受体复合物结合的区域与糖皮质激素受体相互作用，并且包含一个与糖皮质激素受体结合共有序列同源的17个碱基对的序列。RAO 01中该序列的突变可能导致糖皮质激素和雄激素受体结合的丧失。得出的结论是，α2u - 球蛋白基因家族的至少一个成员与雄激素受体复合物直接相互作用，其亲和力与在其他雄激素依赖性基因中观察到的相当。在对糖皮质激素受体也显示出亲和力的区域观察到了这种结合。
