Harlan R E, Garcia M M, Krause J E
Department of Anatomy, Tulane University School of Medicine, New Orleans, Louisiana 70112.
J Comp Neurol. 1989 Sep 8;287(2):179-212. doi: 10.1002/cne.902870204.
To determine the locations of neurons in the rat brain expressing substance P and neurokinin A mRNA, we performed in situ hybridization with a radiolabeled cRNA probe that was complementary to alpha-, beta-, and gamma-preprotachykinin mRNA. Several types of controls indicated specificity of the labeling. Brain regions containing many labeled neurons include the anterior olfactory nucleus, layer II of the olfactory tubercle, the islands of Calleja, the nucleus accumbens, the caudate-putamen, portions of the amygdala and hypothalamus, the medial habenular nucleus, nuclei of the pontine tegmentum, several raphe nuclei, several portions of the reticular formation, and the nucleus of the solitary tract. Less frequent labeled neurons were also found in many other regions of the brain. These results extend many previous immunocytochemical studies of the locations of neurons containing immunoreactive substance P, neurokinin A, and neuropeptide K.
为了确定大鼠脑中表达P物质和神经激肽A mRNA的神经元的位置，我们用与α-、β-和γ-前速激肽原mRNA互补的放射性标记cRNA探针进行了原位杂交。多种对照表明标记具有特异性。含有许多标记神经元的脑区包括前嗅核、嗅结节的II层、Calleja岛、伏隔核、尾状-壳核、杏仁核和下丘脑的部分区域、内侧缰核、脑桥被盖核、几个中缝核、网状结构的几个部分以及孤束核。在脑的许多其他区域也发现了较少的标记神经元。这些结果扩展了先前许多关于含有免疫反应性P物质、神经激肽A和神经肽K的神经元位置的免疫细胞化学研究。
