Vandenbark A A, Hashim G, Offner H
Neuroimmunology Research Laboratory, Veterans Affairs Medical Center, Portland, Oregon 97201.
Nature. 1989 Oct 12;341(6242):541-4. doi: 10.1038/341541a0.
T cells expressing the alpha beta T-cell receptor (TCR) for antigen can elicit anti-idiotypic antibodies specific for the TCR that regulate T-cell function. Defined sequences of the TCR, however, have not been used to elicit specific antibodies and the role of cellular immunity directed against TCR determinants has not been studied. We immunized Lewis rats with a synthetic peptide representing a hypervariable region of the TCR V beta 8 molecule. Subsequent induction of experimental autoimmune encephalomyelitis, a paralytic disease of the central nervous system mediated primarily by V beta 8+ T cells specific for myelin basic protein was prevented. T cells specific for the TCR V beta 8 peptide conferred passive protection against the disease to naive rats, apparently by shifting the predominant T-cell response away from the major encephalitogenic epitope of basic protein. This is the first report demonstrating the use of a synthetic TCR V-region peptide to induce specific regulatory immunity and has important implications for the regulation of human disease characterized by common TCR V-gene usage.
表达针对抗原的αβT细胞受体（TCR）的T细胞可引发针对调节T细胞功能的TCR的抗独特型抗体。然而，TCR的特定序列尚未用于引发特异性抗体，针对TCR决定簇的细胞免疫作用也尚未得到研究。我们用代表TCR Vβ8分子高变区的合成肽免疫Lewis大鼠。随后预防了实验性自身免疫性脑脊髓炎，这是一种主要由针对髓鞘碱性蛋白的Vβ8 + T细胞介导的中枢神经系统麻痹性疾病。针对TCR Vβ8肽的T细胞赋予未感染大鼠对该疾病的被动保护，显然是通过将主要的T细胞反应从碱性蛋白的主要致脑炎性表位转移开。这是第一份证明使用合成TCR V区肽诱导特异性调节免疫的报告，对以常见TCR V基因使用为特征的人类疾病的调节具有重要意义。
