Mercer J E, Clements J A, Funder J W, Clarke I J
Medical Research Centre, Prince Henry's Hospital Campus, Monash Medical Centre, Melbourne, Australia.
Neuroendocrinology. 1989 Sep;50(3):321-6. doi: 10.1159/000125240.
The effect of gonadotropin-releasing hormone (GnRH) and/or estradiol (E2) on pituitary messenger ribonucleic acid (mRNA) levels of luteinizing hormone beta (LH beta), follicle-stimulating hormone beta (FSH beta) and the common alpha-subunit were determined in anterior pituitary glands from ovariectomized (OVX) ewes. Hypothalamo-pituitary disconnected (HPD) ewes receiving appropriate hormonal treatment were used to assess the relative roles of GnRH and E2 in directly regulating FSH beta and alpha-subunit mRNA levels. Levels of LH beta mRNA were increased in OVX animals compared with intact controls, and E2 treatment of OVX animals significantly reduced mRNA levels of LH beta and FSH beta. HPD substantially reduced FSH beta and alpha-subunit mRNA levels. Treatment of OVX/HPD animals with pulses of GnRH (250 ng/2 h) for 1 week restored FSH beta and alpha-subunit mRNA to OVX levels. Combined GnRH and E2 treatment significantly lowered FSH beta mRNA levels, but resulted in a rise in alpha-subunit mRNA levels. Treatment of OVX/HPD ewes with E2 alone had no effect on FSH beta and alpha-subunit mRNA levels. These findings indicate that E2 acts directly on the pituitary to negatively regulate FSH beta mRNA levels, and to positively regulate alpha-subunit mRNA levels in the presence of GnRH.
在去卵巢（OVX）母羊的垂体前叶中，测定了促性腺激素释放激素（GnRH）和/或雌二醇（E2）对促黄体生成素β（LHβ）、促卵泡生成素β（FSHβ）以及共同α亚基的垂体信使核糖核酸（mRNA）水平的影响。使用接受适当激素治疗的下丘脑 - 垂体分离（HPD）母羊来评估GnRH和E2在直接调节FSHβ和α亚基mRNA水平中的相对作用。与完整对照组相比，OVX动物的LHβmRNA水平升高，对OVX动物进行E2治疗可显著降低LHβ和FSHβ的mRNA水平。HPD可大幅降低FSHβ和α亚基的mRNA水平。对OVX/HPD动物以GnRH脉冲（250 ng/2小时）治疗1周可使FSHβ和α亚基mRNA恢复至OVX水平。GnRH和E2联合治疗可显著降低FSHβmRNA水平，但导致α亚基mRNA水平升高。单独用E2治疗OVX/HPD母羊对FSHβ和α亚基mRNA水平无影响。这些发现表明，E2直接作用于垂体，对FSHβmRNA水平起负调节作用，并在存在GnRH的情况下对α亚基mRNA水平起正调节作用。
