Schartl M, Holstein T, Robertson S M, Barnekow A
Gene Center, Max-Planck-Institute for Biochemistry, Martinsried, Federal Republic of Germany.
Oncogene. 1989 Oct;4(10):1185-91.
It has been suggested that the proto-oncogene c-src plays a functional role in developing neurons, and in the mature nerve cells of higher vertebrates. The coelenterate Hydra represents the most primitive known organism possessing nerve cells. With Southern blot hybridizations we have demonstrated src-related sequences in Hydra. Antisera specific for the c-src gene product (pp60c-src) of birds and mammals precipitate a protein from Hydra cell extracts with a tyrosine-specific protein kinase activity. Studies of tissues and cells fractionated from a temperature sensitive mutant of Hydra which is depleted of interstitial (including nerve) cells at the non-permissive temperature, have indicated the src-like kinase of Hydra to be preferentially expressed in nerve cells. The high conservation of structural features and of the expression pattern indicates a basic function for pp60c-src in neurons.
有人提出原癌基因c-src在发育中的神经元以及高等脊椎动物的成熟神经细胞中发挥功能作用。腔肠动物水螅是已知拥有神经细胞的最原始生物。通过Southern印迹杂交，我们在水螅中证实了与src相关的序列。针对鸟类和哺乳动物c-src基因产物（pp60c-src）的特异性抗血清能从水螅细胞提取物中沉淀出一种具有酪氨酸特异性蛋白激酶活性的蛋白质。对来自水螅温度敏感突变体的组织和细胞进行分级分离研究，该突变体在非允许温度下会耗尽间质细胞（包括神经细胞），结果表明水螅的src样激酶在神经细胞中优先表达。结构特征和表达模式的高度保守表明pp60c-src在神经元中具有基本功能。
