希腊市售干葡萄中黄曲霉毒素 B1 和赭曲霉毒素 A 的发生情况。
Occurrence of aflatoxin B1 and ochratoxin A in dried vine fruits from Greek market.
机构信息
a Department of Food Chemistry, Faculty of Chemistry , National and Kapodistrian University of Athens , Athens , Greece.
出版信息
Food Addit Contam Part B Surveill. 2014;7(1):11-6. doi: 10.1080/19393210.2013.825647. Epub 2013 Aug 27.
Twenty-six samples of dried vine fruits from Athens and Thebes (Central Greece) market were simultaneously extracted and cleaned up by immunoaffinity columns and analysed for aflatoxin B1 (AFB1) and ochratoxin A (OTA). A combination of ELISA and HPLC methods was applied for the determination of AFB1. Recovery was 97.6%, RSD 6.46%, while the limit of detection (LOD) and limit of quantification (LOQ) were 0.05 μg kg(-1) and 0.09 μg kg(-1), respectively. OTA concentrations were only estimated by ELISA. Results revealed the presence of AFB1 in 23% of the samples (mean 1.4 μg AFB1 kg(-1)), but none exceeded the EU limit (2 μg AFB1 kg(-1)). However, OTA was detected in 100% of the samples (mean 47.2 μg OTA kg(-1)). Six samples were found to be contaminated at high levels (median 120.6 μg OTA kg(-1)) and 18 exceeded the EU limit (10 μg OTA kg(-1)).
26 份来自雅典和底比斯（希腊中部）市场的干葡萄样本通过免疫亲和柱同时提取和净化，并分析黄曲霉毒素 B1（AFB1）和赭曲霉毒素 A（OTA）。应用 ELISA 和 HPLC 方法组合测定 AFB1。回收率为 97.6%，RSD 为 6.46%，而检测限（LOD）和定量限（LOQ）分别为 0.05μgkg(-1)和 0.09μgkg(-1)。OTA 浓度仅通过 ELISA 进行估计。结果表明，23%的样本中存在 AFB1（平均 1.4μgAFB1kg(-1)），但均未超过欧盟限量（2μgAFB1kg(-1)）。然而，100%的样本中均检测到 OTA（平均 47.2μgOTAkg(-1)）。有 6 个样本被发现受到高浓度污染（中位数 120.6μgOTAkg(-1)），18 个样本超过了欧盟限量（10μgOTAkg(-1)）。
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