Donthaisong Chalermlap, Arunsan Patpicha, Suwannatrai Kulwadee, Prasopdee Sattrachai, Kulsantiwong Jutharat, Wongmaneeprateep Sutee, Suwannatrai Apiporn, Tesana Smarn
Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand.
Acta Trop. 2014 Aug;136:118-22. doi: 10.1016/j.actatropica.2014.04.022. Epub 2014 Apr 26.
Opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the Greater Mekong subregion including Lao PDR, Cambodia, Vietnam and Thailand. Humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. This study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24mm) of Barbonymus gonionotus fish on infection success of O. viverrini cercariae. Additionally, the site of cyst formation of O. viverrini cercariae and subsequent development to the metacercariae was examined. The result showed that cercarial infection dose (F4,95=8.52, P<0.001) age (P<0.001, ƛLR(2)=954.72) and size (P<0.001, ƛLR(2)=1204.84) were significantly associated with number of O. viverrini metacercariae recovery with in every additional fish age, the O. viverrini metacercarial recovery rate increased by 5.4% (P<0.001). Most metacercariae were found in the body of the fish (81.4%). We concluded that O. viverrini cercarial infection dosage, and age and size of fish were important determinants for a successful infection to fish.
猫后睾吸虫是一个重要的公共卫生问题，是包括老挝、柬埔寨、越南和泰国在内的大湄公河次区域胆管癌的主要病因。人类通过食用含有感染性尾蚴的生的、发酵的或部分煮熟的淡水鲤科鱼类而感染。本研究考察了苏氏野鲮鱼的尾蚴感染剂量（25 - 200条尾蚴）、年龄（1 - 60天）和大小（1 - 24毫米）对猫后睾吸虫尾蚴感染成功率的影响。此外，还研究了猫后睾吸虫尾蚴的囊蚴形成部位及其随后发育为后尾蚴的情况。结果表明，尾蚴感染剂量（F4,95 = 8.52，P < 0.001）、年龄（P < 0.001，ƛLR(2) = 954.72）和大小（P < 0.001，ƛLR(2) = 1204.84）与猫后睾吸虫后尾蚴回收数量显著相关，每增加一龄鱼，猫后睾吸虫后尾蚴回收率提高5.4%（P < 0.001）。大多数后尾蚴发现于鱼体（81.4%）。我们得出结论，猫后睾吸虫尾蚴感染剂量以及鱼的年龄和大小是鱼类成功感染的重要决定因素。
