Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000 Zagreb, Croatia.
Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000 Zagreb, Croatia.
Ecotoxicol Environ Saf. 2014 Jul;105:13-21. doi: 10.1016/j.ecoenv.2014.03.035. Epub 2014 May 7.
The visualisation of DNA damage response proteins enables the indirect measurement of DNA damage. Soon after the occurrence of a DNA double-strand break (DSB), the formation of γ-H2AX histone variants is to be expected. This review is focused on the potential use of the γ-H2AX foci assay in assessing the genotoxicity of environmental contaminants including cytostatic pharmaceuticals, since standard methods may not be sensitive enough to detect the damaging effect of low environmental concentrations of such drugs. These compounds are constantly released into the environment, potentially representing a threat to water quality, aquatic organisms, and, ultimately, human health. Our review of the literature revealed that this method could be used in the biomonitoring and risk assessment of aquatic systems affected by wastewater from the production, usage, and disposal of cytostatic pharmaceuticals.
DNA 损伤反应蛋白的可视化使得 DNA 损伤的间接测量成为可能。在双链 DNA 断裂 (DSB) 发生后不久，γ-H2AX 组蛋白变体的形成是可以预期的。本综述重点介绍了 γ-H2AX 焦点检测法在评估环境污染物（包括细胞毒性药物）遗传毒性方面的潜在应用，因为标准方法可能不够灵敏，无法检测到此类药物在低环境浓度下的损害作用。这些化合物不断释放到环境中，可能对水质、水生生物以及最终对人类健康构成威胁。我们对文献的综述表明，该方法可用于监测受生产、使用和处理细胞毒性药物的废水影响的水生系统的生物监测和风险评估。
