酿酒酵母 Rpb4 参与与转录延伸相关的途径的子集。

Involvement of S. cerevisiae Rpb4 in subset of pathways related to transcription elongation.

机构信息

Dept. of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.

出版信息

Gene. 2014 Jul 15;545(1):126-31. doi: 10.1016/j.gene.2014.04.061. Epub 2014 Apr 27.

DOI:10.1016/j.gene.2014.04.061

Abstract

Yeast Rpb4, a subunit of RNA pol II is not essential for viability but is involved in multiple cellular phenotypes such as temperature sensitivity, enhanced pseudohyphal morphology, and decreased sporulation. Both in vivo and in vitro studies strongly support involvement of Rpb4 in transcription initiation, while its role in transcription elongation is not entirely consistent. Here we show that Rpb4 is not required for recruitment of RNA pol II on the coding region of YLR454w, a representative long gene. Yet we find strong genetic interaction of rpb4∆ with mutants in many transcription elongation factors such as Paf1, Spt4, Dst1, Elp3 and Rpb9. We demonstrate that, Rpb4 interacts functionally with Paf1 to affect the transcription elongation of the FKS1 gene. Our results suggest that while Rpb4 is not required for general transcription elongation, it could support transcription elongation for specific of class of genes by interaction with other elongation factors.

摘要

酵母 RNA 聚合酶 II 的 Rpb4 亚基不是生存所必需的，但参与多种细胞表型，如温度敏感性、增强的假菌丝形态和减少的孢子形成。体内和体外研究都强烈支持 Rpb4 参与转录起始，而其在转录延伸中的作用并不完全一致。在这里，我们表明 Rpb4 不需要招募 RNA pol II 在编码区的 YLR454w，一个代表性的长基因。然而，我们发现 rpb4∆与许多转录延伸因子（如 Paf1、Spt4、Dst1、Elp3 和 Rpb9）的突变体之间存在强烈的遗传相互作用。我们证明，Rpb4 与 Paf1 相互作用以影响 FKS1 基因的转录延伸。我们的结果表明，虽然 Rpb4 不是一般转录延伸所必需的，但它可以通过与其他延伸因子的相互作用来支持特定基因类别的转录延伸。