基于报告分子触发的等温指数扩增策略用于超灵敏均相无标记电化学核酸生物传感
Reporter-triggered isothermal exponential amplification strategy in ultrasensitive homogeneous label-free electrochemical nucleic acid biosensing.
作者信息
Nie Ji, Zhang De-Wen, Zhang Fang-Ting, Yuan Fang, Zhou Ying-Lin, Zhang Xin-Xiang
机构信息
Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China.
出版信息
Chem Commun (Camb). 2014 Jun 14;50(47):6211-3. doi: 10.1039/c4cc00475b. Epub 2014 Apr 30.
A simple and novel reporter-triggered isothermal exponential amplification reaction (R-EXPAR) integrated with a miniaturized electrochemical device was developed, which achieved excellent improvement (five orders of magnitude) of sensitivity toward reporter, G-quadruplex. This R-EXPAR strategy has been successfully implemented to construct a homogeneous label-free electrochemical sensor for ultrasensitive DNA detection.
开发了一种简单新颖的基于报告分子触发的等温指数扩增反应（R-EXPAR），并与小型化电化学装置集成，该反应对报告分子G-四链体的灵敏度实现了显著提高（五个数量级）。这种R-EXPAR策略已成功用于构建用于超灵敏DNA检测的均相无标记电化学传感器。
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