Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU) , Tokyo , Japan.
Front Bioeng Biotechnol. 2015 Mar 5;3:29. doi: 10.3389/fbioe.2015.00029. eCollection 2015.
Nucleic acid amplification is a gold standard technique for analyzing a tiny amount of nucleotides in molecular biology, clinical diagnostics, food safety, and environmental testing. Electrical and electrochemical monitoring of the amplification process draws attention over conventional optical methods because of the amenability toward point-of-care applications as there is a growing demand for nucleic acid sensing in situations outside the laboratory. A number of electrical and electrochemical techniques coupled with various amplification methods including isothermal amplification have been reported in the last 10 years. In this review, we highlight recent developments in the electrical and electrochemical monitoring of nucleic acid amplification.
核酸扩增是分子生物学、临床诊断、食品安全和环境检测中分析微量核苷酸的金标准技术。在临床检测中，对扩增过程进行电和电化学监测受到了越来越多的关注，因为它比传统的光学方法更适用于即时检测应用，而对核酸检测的需求也在不断增加。在过去的 10 年中，已经报道了许多与各种扩增方法（包括等温扩增）相结合的电和电化学技术。在本文综述中，我们重点介绍了核酸扩增电和电化学监测的最新进展。
