MPP⁺对大鼠纹状体中5-羟色胺和5-羟吲哚乙酸体内释放的影响。

Effects of MPP+ on the release of serotonin and 5-hydroxyindoleacetic acid from rat striatum in vivo.

作者信息

Miyake H, Chiueh C C

机构信息

Laboratory of Cerebral Metabolism, National Institute of Mental Health, Bethesda, MD 20892.

出版信息

Eur J Pharmacol. 1989 Jul 4;166(1):49-55. doi: 10.1016/0014-2999(89)90682-1.

DOI:10.1016/0014-2999(89)90682-1

PMID:2478372

Abstract

A procedure utilizing brain dialysis was employed to investigate the effects of 1-methyl-4-phenylpyridinium ion (MPP+), the major metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), on the in vivo release of 5-hydroxytryptamine (5-HT) from the rat striatum. MPP+ (10(-5)-10(-2) M) was administered through the microdialysis probe and dialysates were collected and assayed for monoamines and their metabolites by a high performance liquid chromatography coupled with an electrochemical detector (HPLC-EC). In addition to a dopamine (DA) releasing action, MPP+ caused a cumulatively dose-dependent increase in the release of 5-HT while concomitantly producing a decrease in the efflux of 5-hydroxyindoleacetic acid (5-HIAA). This MPP+-induced increase in the 5-HT release, may play an important role in the acute 5-HT syndrome seen in animals after MPTP.

摘要

采用脑透析程序研究1-甲基-4-苯基吡啶离子（MPP+），即1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）的主要代谢产物，对大鼠纹状体中5-羟色胺（5-HT）体内释放的影响。通过微透析探针给予MPP+（10^-5 - 10^-2 M），收集透析液，并用高效液相色谱-电化学检测器（HPLC-EC）分析单胺及其代谢产物。除了具有释放多巴胺（DA）的作用外，MPP+还导致5-HT释放呈累积剂量依赖性增加，同时使5-羟吲哚乙酸（5-HIAA）流出减少。MPP+诱导的5-HT释放增加可能在MPTP处理后的动物急性5-HT综合征中起重要作用。