Moynihan J, Burstyn D, Zauderer M
Cancer Center, University of Rochester Medical Center, New York 14642.
Immunology. 1989 Oct;68(2):199-203.
Cloned autoreactive T-cell lines and hybridomas have been selected in many laboratories. A number of observations have suggested that activation of Ia-positive stimulators may be required for optimal induction of an autoreactive response. We have examined the ability of small resting B cells fractionated by centrifugal elutriation to stimulate the proliferative response of seven cloned autoreactive T-cell lines. Of these, six were efficiently stimulated by resting B cells. One I-Ed-specific Th2-type T-cell clone failed to be stimulated by resting B cells. This clone did, however, respond to this same cell fraction following lipopolysaccharide (LPS) activation. An independent I-E-specific Th2 clone was stimulated by resting B cells. It appears, therefore, that a requirement for activated stimulators is not a general property of either autoreactive T cells or the Th2 helper T-cell subset.
许多实验室已筛选出克隆的自身反应性T细胞系和杂交瘤。一系列观察结果表明，Ia阳性刺激细胞的激活可能是最佳诱导自身反应性应答所必需的。我们检测了通过离心淘析分离的静止小B细胞刺激7个克隆的自身反应性T细胞系增殖反应的能力。其中，6个能被静止B细胞有效刺激。一个I-Ed特异性Th2型T细胞克隆不能被静止B细胞刺激。然而，该克隆在脂多糖（LPS）激活后对同一细胞组分有反应。一个独立的I-E特异性Th2克隆能被静止B细胞刺激。因此，似乎对激活的刺激细胞的需求既不是自身反应性T细胞也不是Th2辅助性T细胞亚群的普遍特性。
