Sun D, Klinkert W E
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38101.
J Immunol. 1989 Nov 1;143(9):2867-72.
Inoculation of Lewis rats with live or attenuated (irradiated or paraformaldehyde-fixed) CD4+ encephalitogenic T cells (S1 line) protects the recipients from transferred experimental autoimmune encephalomyelitis (tEAE) induced by S1 cells. A CD8+ T lymphocyte population specifically activated against the EAE-inducing S1 cells can be readily isolated from the lymphoid organs of pretreated animals. We show, in the present study, that encephalitogenic T cell lines derived from Lewis rats differ in their ability to induce resistance against tEAE in vivo and to stimulate CD8+ cell proliferation in vitro. We also demonstrate that the S19 line of encephalitogenic T cells, in combination with myelin basic protein (MBP), can stimulate CD8+ cell proliferation in vitro. The CD8+ cells generated in this way strongly suppress MBP-specific T cell proliferation in vitro. This combined effect of T cells and MBP was also evident in vivo. Neither S19 cells nor MBP alone induced resistance against S19-mediated tEAE, rather coinjection of these cells and MBP was required. Our results suggest that resistance to EAE is mediated by distinct populations of encephalitogenic T cells that activate Ts cells through different mechanisms. In some instances, both autoreactive T cells and their relevant autoantigen(s) may be needed to activate Ts cells in vivo.
用活的或减毒的（经辐射或多聚甲醛固定的）CD4+致脑炎性T细胞（S1系）接种Lewis大鼠，可使受体免受S1细胞诱导的转移性实验性自身免疫性脑脊髓炎（tEAE）的侵害。可以很容易地从预处理动物的淋巴器官中分离出针对诱导EAE的S1细胞特异性激活的CD8+ T淋巴细胞群体。在本研究中，我们表明，源自Lewis大鼠的致脑炎性T细胞系在体内诱导抗tEAE抗性以及在体外刺激CD8+细胞增殖的能力方面存在差异。我们还证明，致脑炎性T细胞的S19系与髓鞘碱性蛋白（MBP）结合，可在体外刺激CD8+细胞增殖。以这种方式产生的CD8+细胞在体外强烈抑制MBP特异性T细胞增殖。T细胞和MBP的这种联合作用在体内也很明显。单独的S19细胞或MBP都不能诱导对S19介导的tEAE的抗性，而是需要将这些细胞与MBP共同注射。我们的结果表明，对EAE的抗性是由不同群体的致脑炎性T细胞介导的，这些细胞通过不同机制激活Ts细胞。在某些情况下，自身反应性T细胞及其相关自身抗原可能都需要在体内激活Ts细胞。
