Dias Felipe F, Zarantonello Victor C, Parreira Gleydes G, Chiarini-Garcia Hélio, Melo Rossana C N
1Laboratory of Cellular Biology,Department of Biology,Federal University of Juiz de Fora (UFJF),Juiz de Fora,MG 36036-900,Brazil.
2Laboratory of Structural Biology and Reproduction,Federal University of Minas Gerais (UFMG),Belo Horizonte,MG 31270-901,Brazil.
Microsc Microanal. 2014 Jun;20(3):869-78. doi: 10.1017/S143192761400066X. Epub 2014 May 1.
Macrophages are widely distributed immune system cells with essential functions in tissue homeostasis, apoptotic cell clearance, and first defense in infections. A distinguishing feature of activated macrophages participating in different situations such as inflammatory and metabolic diseases is the presence of increased numbers of lipid-rich organelles, termed lipid bodies (LBs) or lipid droplets, in their cytoplasm. LBs are considered structural markers of activated macrophages and are involved in different functions such as lipid metabolism, intracellular trafficking, and synthesis of inflammatory mediators. In this review, we revisit the distinct morphology of LB organelles actively formed within macrophages in response to infections and cell clearance, taking into account new insights provided by ultrastructural studies. We also discuss the LB interactions within macrophages, revealed by transmission electron microscopy, with a focus on the remarkable LB-phagosome association and discuss potential links between structural aspects and function.
巨噬细胞是广泛分布的免疫系统细胞，在组织稳态、凋亡细胞清除以及感染的初始防御中发挥着重要作用。参与炎症和代谢疾病等不同情况的活化巨噬细胞的一个显著特征是其细胞质中富含脂质的细胞器数量增加，这些细胞器被称为脂质体（LBs）或脂滴。脂质体被认为是活化巨噬细胞的结构标志物，并参与脂质代谢、细胞内运输和炎症介质合成等不同功能。在本综述中，我们结合超微结构研究提供的新见解，重新审视巨噬细胞内响应感染和细胞清除而活跃形成的脂质体细胞器的独特形态。我们还讨论了通过透射电子显微镜揭示的巨噬细胞内脂质体的相互作用，重点关注显著的脂质体-吞噬体关联，并探讨结构方面与功能之间的潜在联系。
