合成染料偶联物以使用荧光显微镜观察癌细胞。
Synthesis of dye conjugates to visualize the cancer cells using fluorescence microscopy.
作者信息
Pu Yang, Tang Rui, Xue Jianpeng, Wang W B, Xu Baogang, Achilefu S
出版信息
Appl Opt. 2014 Apr 10;53(11):2345-51. doi: 10.1364/AO.53.002345.
Abstract
The clinical diagnosis of most cancers is based on evaluation of histology microscopic slides to view the size and shape of cellular nuclei and morphological structure of tissue. To achieve this goal for in vivo and in-deep tissues, near infrared dyes-bovine serum albumin and immunoglobulin G conjugates were synthesized. The spectral study shows that the absorption and fluorescence of the dye conjugates are in the "tissue optical window" spectral ranges between 650 and 900 nm. The internalization and pinocytosis of the synthesized compounds were investigated at cell level using fluorescence microscopy to obtain the optimal concentration and staining time.
摘要
大多数癌症的临床诊断是基于对组织学显微镜载玻片的评估，以观察细胞核的大小和形状以及组织的形态结构。为了在体内和深部组织中实现这一目标，合成了近红外染料-牛血清白蛋白和免疫球蛋白G缀合物。光谱研究表明，染料缀合物的吸收和荧光在650至900nm的“组织光学窗口”光谱范围内。使用荧光显微镜在细胞水平上研究了合成化合物的内化和胞饮作用，以获得最佳浓度和染色时间。
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