a Department of Biotechnology , Institute of Bioresources and Sustainable Development, Government of India, Takyelpat Institutional Area , Imphal 795001 , Manipur , India.
Nat Prod Res. 2014;28(18):1503-6. doi: 10.1080/14786419.2014.913241. Epub 2014 Apr 30.
Harringtonine (HT) and homoharringtonine (HHT) are Cephalotaxus alkaloids with considerable antileukaemic activity. The objectives of this research were to (1) determine the content of HT and HHT present in Cephalotaxus griffithii needles alkaloid fraction (CGAF) and (2) compare the antiproliferative activity of CGAF, with that of HT and HHT on chronic myelogenous leukaemia K562 cell. The concentration of HT and HHT was found to be 122.14 and 16.79 mg/g of CGAF, respectively. Treatment of K562 cells with CGAF, HT and HHT decreased the viable cells in a dose- and time-dependent manner. Interestingly, the maximum cell death was found in CGAF, with IC50 value which was 3- to 4.6-fold lower than those of HT and HHT. Our results indicate that HT content in the needles of C. griffithii is higher than HHT, and alkaloids other than HT and HHT in CGAF are predominantly responsible for K562 cell death.
三尖杉酯碱（HT）和高三尖杉酯碱（HHT）是具有相当抗白血病活性的 Cephalotaxus 生物碱。本研究的目的是：（1）测定 Cephalotaxus griffithii 针叶生物碱部分（CGAF）中 HT 和 HHT 的含量；（2）比较 CGAF 与 HT 和 HHT 对慢性髓性白血病 K562 细胞的增殖抑制活性。结果发现，HT 和 HHT 在 CGAF 中的浓度分别为 122.14 和 16.79 mg/g。CGAF、HT 和 HHT 处理 K562 细胞可呈剂量和时间依赖性降低活细胞数。有趣的是，CGAF 引起的最大细胞死亡，其 IC50 值比 HT 和 HHT 低 3-4.6 倍。我们的结果表明，C. griffithii 针叶中的 HT 含量高于 HHT，而 CGAF 中除 HT 和 HHT 以外的生物碱主要负责 K562 细胞死亡。
