Gurung Ratna B, Begg Douglas J, Purdie Auriol C, Bach Horacio, Whittington Richard J
Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia.
Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, BC, Canada.
Vet Immunol Immunopathol. 2014 Jul 15;160(1-2):129-32. doi: 10.1016/j.vetimm.2014.03.013. Epub 2014 Apr 3.
Evasion of host defense mechanisms and survival inside infected host macrophages are features of pathogenic mycobacteria including Mycobacterium avium subspecies paratuberculosis, the causative agent of Johne's disease in ruminants. Protein tyrosine phosphatase A (PtpA) has been identified as a secreted protein critical for survival of mycobacteria within infected macrophages. The host may mount an immune response to such secreted proteins. In this study, the humoral immune response to purified recombinant M. avium subsp. paratuberculosis PtpA was investigated using sera from a cohort of sheep infected with M. avium subsp. paratuberculosis and compared with uninfected healthy controls. A significantly higher level of reactivity to PtpA was observed in sera collected from M. avium subspecies paratuberculosis infected sheep when compared to those from uninfected healthy controls. PtpA could be a potential candidate antigen for detection of humoral immune responses in sheep infected with M. avium subspecies paratuberculosis.
逃避宿主防御机制并在受感染的宿主巨噬细胞内存活是包括副结核分枝杆菌（反刍动物约翰氏病的病原体）在内的致病性分枝杆菌的特征。蛋白酪氨酸磷酸酶A（PtpA）已被确定为分枝杆菌在受感染巨噬细胞内存活所必需的一种分泌蛋白。宿主可能会对这类分泌蛋白产生免疫反应。在本研究中，利用一组感染副结核分枝杆菌的绵羊血清，研究了对纯化重组副结核分枝杆菌PtpA的体液免疫反应，并与未感染的健康对照进行比较。与未感染的健康对照相比，从感染副结核分枝杆菌的绵羊采集的血清中观察到对PtpA的反应性水平显著更高。PtpA可能是检测感染副结核分枝杆菌绵羊体液免疫反应的潜在候选抗原。
