Khare M, Mohanty C, Das B K, Jyoti A, Mukhopadhyay B, Mishra S P
Department of Anatomy, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Department of Pediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Int J Pediatr. 2014;2014:254396. doi: 10.1155/2014/254396. Epub 2014 Mar 27.
Background/Objectives. The aim of this study was to evaluate oxidant and antioxidant status in children with different grades of Protein Energy Malnutrition (PEM). Subjects/Methods. A total of two hundred fifty (250) children (age range: 6 months to 5 years) living in eastern UP, India, were recruited. One hundred and ninety-three (193) of these children had different grades of PEM (sixty-five (65) children belong to mild, sixty (60) to moderate, and sixty-eight (68) to severe group). Grading in group was done after standardization in weight and height measurements. Fifty-seven (57) children who are age and and sex matched, healthy, and well-nourished were recruited from the local community and used as controls after checking their protein status (clinical nutritional status) with height and weight standardization. Redox homeostasis was assessed using spectrophotometric/colorimetric methods. Results. In our study, erythrocyte glutathione (GSH), plasma Cu, Zn-superoxide dismutase (Cu,Zn-SOD,EC 1.15.1.1), ceruloplasmin (Cp), and ascorbic acid were significantly (P < 0.001) more decreased in children with malnutrition than controls. Plasma malondialdehyde (MDA), and protein carbonyl (PC) were significantly (P < 0.001) raised in cases as compared to controls. Conclusion. Stress is created as a result of PEM which is responsible for the overproduction of reactive oxygen species (ROSs). These ROSs will lead to membrane oxidation and thus an increase in lipid peroxidation byproducts such as MDA and protein oxidation byproducts such as PC mainly. Decrease in level of antioxidants suggests an increased defense against oxidant damage. Changes in oxidant and antioxidant levels may be responsible for grading in PEM.
背景/目的。本研究旨在评估不同程度蛋白质能量营养不良（PEM）儿童的氧化应激和抗氧化状态。对象/方法。共招募了250名居住在印度北方邦东部的儿童（年龄范围：6个月至5岁）。其中193名儿童患有不同程度的PEM（65名儿童属于轻度，60名属于中度，68名属于重度组）。根据体重和身高测量标准化对组内进行分级。从当地社区招募了57名年龄、性别匹配、健康且营养良好的儿童，在通过身高和体重标准化检查其蛋白质状态（临床营养状况）后用作对照。使用分光光度法/比色法评估氧化还原稳态。结果。在我们的研究中，营养不良儿童的红细胞谷胱甘肽（GSH）、血浆铜锌超氧化物歧化酶（Cu,Zn-SOD，EC 1.15.1.1）、铜蓝蛋白（Cp）和抗坏血酸显著（P < 0.001）低于对照组。与对照组相比，病例组血浆丙二醛（MDA）和蛋白质羰基（PC）显著（P < 0.001）升高。结论。PEM导致应激，这是活性氧（ROS）过度产生的原因。这些ROS会导致膜氧化，从而主要导致脂质过氧化产物如MDA增加以及蛋白质氧化产物如PC增加。抗氧化剂水平降低表明对氧化损伤的防御增强。氧化应激和抗氧化剂水平的变化可能与PEM的分级有关。
