Jakhesara Subhash J, Bhatt Vaibhav D, Patel Namrata V, Prajapati Kantilal S, Joshi Chaitanya G
Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388001 India.
Department of Veterinary Pathology, College of Veterinary Science & Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388001 India.
Springerplus. 2014 Apr 18;3:196. doi: 10.1186/2193-1801-3-196. eCollection 2014.
The present study reports isolation and characterization of H9N2 virus responsible for disease characterized by symptoms including difficulty in respiration, head swelling, nasal discharge, reduced feed intake, cyanotic comb, reduced egg production and mortality. Virus isolation from allantoic fluid inoculated with tracheal aspirates and whole genome sequencing of two isolates were performed on an Ion-Torrent sequencer. Phylogenetic analysis revealed that the two H9N2 isolates are reassortant viruses showing a G1-like lineage for HA, NA and NP, a Hok/49/98-like lineage for PB1 and PA, PK/UDL-01/05-like lineage for PB2, IL/90658/00-like lineage for NS and an unknown lineage for M gene. Analyses of the HA cleavage site showed a sequence of (333PARSSR↓GL340) indicating that these isolates are of low pathogenicity. Isolate 2 has leucine at amino acid position 226, a substitution which is associated with mammalian adaptation of avian influenza virus. Isolate 1 has the S31N substitution in the M2 gene that has been associated with drug resistance as well as R57Q and C241Y mutations in the NP gene which are associated with human adaptation. The result reported here gives deep insight in to H9N2 viruses circulating in domestic poultry of India and supports the policy of active efforts to control and manage H9N2 infections in Indian poultry.
本研究报告了对导致疾病的H9N2病毒的分离和鉴定，该疾病的症状包括呼吸困难、头部肿胀、流鼻涕、采食量减少、鸡冠发绀、产蛋量下降和死亡率。对接种气管吸出物的尿囊液进行病毒分离，并在Ion-Torrent测序仪上对两个分离株进行全基因组测序。系统发育分析表明，这两个H9N2分离株是重配病毒，其HA、NA和NP呈现G1样谱系，PB1和PA呈现Hok/49/98样谱系，PB2呈现PK/UDL-01/05样谱系，NS呈现IL/90658/00样谱系，M基因呈现未知谱系。对HA裂解位点的分析显示序列为(333PARSSR↓GL340)，表明这些分离株具有低致病性。分离株2在氨基酸位置226处为亮氨酸，该取代与禽流感病毒的哺乳动物适应性有关。分离株1在M2基因中有S31N取代，该取代与耐药性有关，在NP基因中有R57Q和C241Y突变，这些突变与人类适应性有关。此处报告的结果深入洞察了印度家禽中传播的H9N2病毒，并支持积极努力控制和管理印度家禽中H9N2感染的政策。
