Liu Lin-Yu, Jiang Shuang-Ying, Wang Li-Jie, Yi Hu, Zhao Sheng-Cang, Tang Zhi-Jian, Xu Cui-Ling, Dong Jie, Gao Rong-Bao, Zhang Ye, Zou Shu-Mei, Li Xiao-Dan, Yang Lei, Yang Jing, Chen Tao, Shu Yue-Long
Bing Du Xue Bao. 2014 Mar;30(2):109-18.
Five H9N2 avian influenza virus strains were isolated from the environmental samples in live poultry market in Qinghai Lake region from July to September, 2012. To evaluate the phylogenetic characteristics of these H9N2 isolates, the eight gene segments were amplified by RT-PCR and sequenced. The phylogenetic and molecular characteristics of the five strains were analyzed. The results showed that the HA genes of five strains shared 93. 2%-99. 1% nucleotide identities with each other, and the NA genes shared 94. 5%-99. 8% nucleotide identities. The HA cleavage site sequence of the A/environment/qinghai/ 017/2012 isolate was PSKSSRGLF, and the HA cleavage site sequences of the other four strains were all PSRSSRGLF. The HA receptor-binding site had the Q226L mutation. The M1 gene segment had the N30D and T215A mutations. The phylogenetic analysis showed that the five strains were similar to the virus A/chicken/Hunan/5260/2005 (H9N2) isolated in Hunan Province, China and were reassortant genotype viruses; the HA, NA, and NS genes belonged to the Y280-like lineage; the MP gene belonged to the G1-like lineage; the NP, PB1, PB2, and PA genes belonged to the F98-like lineage.
2012年7月至9月，从青海湖地区活禽市场的环境样本中分离出5株H9N2禽流感病毒株。为评估这些H9N2分离株的系统发育特征，通过RT-PCR扩增8个基因片段并进行测序。分析了这5株病毒的系统发育和分子特征。结果显示，5株病毒的HA基因彼此间核苷酸同源性为93.2%-99.1%，NA基因核苷酸同源性为94.5%-99.8%。A/环境/青海/017/2012分离株的HA裂解位点序列为PSKSSRGLF，其他4株的HA裂解位点序列均为PSRSSRGLF。HA受体结合位点有Q226L突变。M1基因片段有N30D和T215A突变。系统发育分析表明，这5株病毒与中国湖南省分离的A/鸡/湖南/5260/2005（H9N2）病毒相似，为重组基因型病毒；HA、NA和NS基因属于Y280样谱系；MP基因属于G1样谱系；NP、PB1、PB2和PA基因属于F98样谱系。
